文献类型： 外文期刊

作者： Wang, G. ¹ ; Zhang, J. ¹ ; Song, F. ¹ ; Gu, A. ³ ; Uwais, A. ⁴ ; Shao, T. ¹ ; Huang, D. ² ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

3.Xinjiang Agr Univ, Coll Agr, Urumqi, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi, Peoples R China

关键词： Bacillus thuringiensis;bioassays;nontarget organisms

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 2020影响因子：3.772； 五年影响因子：3.963 ）

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收录情况： SCI

摘要： To construct a recombinant Bacillus thuringiensis (Bt) strain with broad insecticidal spectrum and investigate its impact on nontarget organisms in field. The cry-type gene of wild Bt strain UV17 was identified and a novel cry1Ba gene was cloned. The cry3Aa7 gene, which was highly toxic to coleopteran pests, was introduced into UV17, and a recombinant strain designated as UV173A was obtained. Bioassay results showed that UV173A was not only highly toxic against Plutella xylostella (50% lethal concentration [LC] = 18p"03 og mlp#), but also against coleopteran Leptinotarsa decernlineata (LC = 0p"19 mg mlp#). The recombinant strain was then tested in field trials to monitor its spatial variation of population and to investigate the impact on nontarget invertebrates. A recombinant Bt stain UV173A with broad insecticidal spectrum was obtained, and it did not cause adverse effects on the population of nontarget organisms. The results obtained here indicated that cry1Ba3 gene may be useful for the resistance management of P. xylostella, and the recombinant stain UV173A was potential for field application against some crucifer vegetable pests as well as L. decemlineata.