文献类型： 外文期刊

作者： Ke, Yanguo ¹ ; Ashraf, Umair ² ; Wang, Dongdong ³ ; Hassan, Waseem ⁴ ; Zou, Ying ⁵ ; Qi, Ying ⁵ ; Zhou, Yiwei ⁶ ; Abbas, Farhat ³ ;

作者机构： 1.Kunming Univ, Coll Econ & Management, Yunnan Urban Agr Engn & Technol Res Ctr, Kunming 650208, Peoples R China

2.Univ Educ, Dept Bot, Div Sci & Technol, Lahore 54770, Pakistan

3.South China Agr Univ, Coll Hort, Guangdong Litchi Engn Res Ctr, Key Lab Biol & Genet Improvement Hort Crops South, Guangzhou 510642, Peoples R China

4.Muhammad Nawaz Shareef Univ Agr, Dept Soil & Environm Sci, Multan 60001, Pakistan

5.Kunming Univ, Coll Agron, Yunnan Urban Agr Engn & Technol Res Ctr, Yunnan Key Lab Konjac Biol, Kunming 650214, Peoples R China

6.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Prov Key Lab Ornamental Plant Germplasm, Guangzhou 510640, Peoples R China

7.Coconstruct Minist & Prov, Minist Agr & Rural Affairs, Inst Trop Fruit Trees, Key Lab Genet Resources Evaluat & Utilizat Trop Fr, Haikou 571100, Peoples R China

8.Key Lab Trop Fruit Tree Biol Hainan Prov, Haikou 571100, Peoples R China

关键词： Hydrangea macrophylla; floral color; anthocyanin biosynthesis; metabolomics; transcriptome

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 5 期

页码：

收录情况： SCI

摘要： Hydrangea (Hydrangea macrophylla) is distinguished by having sepals instead of real petals, a trait that facilitates color diversity. Floral color is largely predetermined by structural genes linked to anthocyanin production, but the genetic factors determining floral hue in this non-model plant remain unclear. Anthocyanin metabolites, transcriptome, and the CIEL*a*b* hue system were employed to elucidate the biochemical and molecular mechanisms of floral color formation in three hydrangea cultivars: 'DB' (deep blue), 'LB' (light blue), and 'GB' (green blue). UPLC-MS/MS identified 47 metabolites, with delphinidin, cyanidin, malvidin, petunidin, pelargonidin, and peonidin being prominent. Delphinidins were 90% of the primary component in 'DB'. The dataset identifies 51 and 31 DEGs associated with anthocyanin, flavonoid, and chlorophyll biosynthesis, with CHS, CHI, F3H, F3 ' 5 ' H, DFR, ANS, BZ1, and 3AT displaying the highest expression in 'DB'. Notably, DFR (cluster-46471.3) exhibits high expression in 'DB' while being down-regulated in 'LB' and 'GB', correlating with higher anthocyanin levels in floral pigmentation. Comparative analyses of 'LB' vs. 'DB', 'DB' vs. 'GB', and 'LB' vs. 'GB' revealed 460, 490, and 444 differentially expressed TFs, respectively. WRKY, ERF, bHLH, NAC, and AP2/ERF showed the highest expression in 'DB', aligning with the color formation and key anthocyanin biosynthesis-related gene expression. The findings reveal the molecular mechanisms behind floral pigmentation variations and lay the groundwork for future hydrangea breeding programs.