Shiitake mushroom-derived extracellular nanovesicles: Preparation, characterization, and inhibition of Caco-2 cells
文献类型： 外文期刊
作者： Yang, Xueli 1 ; Zong, Zihao 1 ; Niu, Ben 1 ; Chen, Hangjun 1 ; Wu, Weijie 1 ; Fang, Xiangjun 1 ; Liu, Ruiling 1 ; Gao, Haiyan 1 ; Mu, Honglei 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Food Sci, Key Lab Light Ind Fruit & Vegetable Preservat & Pr, Minist Agr & Rural Affairs,Key Lab Postharvest Veg, Hangzhou 310021, Peoples R China
关键词： Shiitake mushroom; Extracellular nanovesicles; Sucrose gradient ultracentrifugation; Stability; Caco-2 cell
期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）
ISSN： 0308-8146
年卷期： 2025 年 463 卷
页码：
收录情况： SCI
摘要： In this study, Shiitake mushroom-derived extracellular nanovesicles (SMDENVs) were isolated from fresh Shiitake mushrooms by ultracentrifugation and sucrose gradient ultracentrifugation. The morphological characteristics of SMDENVs were investigated via Transmission Electron Microscopy and Laser Scanning Confocal Microscopy. SMDENVs were spherical, hollow, and uniform in size, with an average diameter of 177.6 +/- 51.4 nm. Based on the analysis of lipidomics and proteomics, 383 lipids species and 1290 proteins were identified in SMDENVs. Compared with the conventional liposomes, SMDENVs demonstrated higher stability in different environmental conditions. Furthermore, we observed that SMDENVs were cytocompatible and inhibited the proliferation of Caco-2 cells. SMDENVs could be phagocytized by Caco-2 cells in a time-dependent manner. Further, SMDENVs also inhibited the proliferation of Caco-2 cells in a dose-dependent manner, and the half-maximal inhibitory concentration (IC50) was 236.2 +/- 3.2 mu g/mL. Additionally, SMDENVs induced cellular apoptosis by increasing the levels of reactive oxygen species and decreasing the mitochondrial membrane potential.
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