Defense Responses Stimulated by Bacillus subtilis NCD-2 Through Salicylate- and Jasmonate-Dependent Signaling Pathways Protect Cotton Against Verticillium Wilt
文献类型： 外文期刊
作者： Mo, Shaojing 1 ; Zhao, Weisong 1 ; Wei, Yarui 1 ; Su, Zhenhe 1 ; Li, Shezeng 1 ; Lu, Xiuyun 1 ; Zhang, Xiaoyun 1 ; Qu, Yuanhang 1 ; Wang, Peipei 1 ; Dong, Lihong 1 ; Zhang, Jiaqi 1 ; Guo, Qinggang 1 ; Ma, Ping 1 ;
作者机构： 1.Minist Agr & Rural Affairs China, Hebei Acad Agr & Forestry Sci, Integrated Pest Management Innovat Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Inst Pl, Baoding 071000, Peoples R China
关键词：
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 7 期
页码：
收录情况： SCI
摘要： Bacillus subtilis NCD-2 demonstrates exceptional biocontrol potential against cotton Verticillium wilt. While previous studies have established its direct antifungal activity (e.g., inhibiting Verticillium dahliae mycelial growth and spore germination), our work reveals a novel mechanism: NCD-2 primes systemic resistance in cotton by activating plant immune-signaling pathways. Firstly, transcriptional profiling uncovered that NCD-2 triggers a defense response in roots analogous to V. dahliae infection, allowing cotton to maintain a more balanced state when confronted with pathogen attacks. Meanwhile, the mutant strains triangle fen and triangle srf-defective in lipopeptide synthesis-also improved cotton resistance to Verticillium wilt by activating partially identical defense pathways in cotton roots. Furthermore, the application of lipopeptide compounds derived from NCD-2, particularly surfactin and fengycin, could enhance host resistance to V. dahliae. Using an RT-qPCR approach, we found that numerous resistance-related genes were induced by these lipopeptide compounds. The up-regulation of SA/JA pathway markers (e.g., NPR1, ICS1, COI1, and LOX1) revealed NCD-2's activation of plant immune signaling. Using virus-induced gene silencing (VIGS), we conclusively linked SA and JA signaling to NCD-2-induced defense priming. Silencing either pathway abolished resistance, highlighting their indispensable coordination. By bridging mechanistic insights and agricultural applicability, our work positions NCD-2 as a sustainable alternative to conventional fungicides, addressing both crop productivity and environmental health.
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