Development and Utilization of Quantitative Detection for Probiotics Bacillus velezensis B31 with Potential Protection Against Tomato Fusarium Wilt
文献类型： 外文期刊
作者： Su, Zhenhe 1 ; Gao, Yanan 1 ; Dong, Lemeng 3 ; He, Yunzhuan 2 ; Guo, Qinggang 1 ; Ma, Ping 1 ;
作者机构： 1.Minist Agr & Rural Affairs China, Hebei Acad Agr & Forestry Sci, Integrated Pest Management Ctr Hebei Prov, Plant Protect Inst,Key Lab IPM Crops Northern Reg, 437 Dongguan St, Baoding 071000, Hebei, Peoples R China
2.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Peoples R China
3.Univ Amsterdam, Swammerdam Inst Life Sci, Plant Hormone Biol Grp, Sci Pk 904, NL-1098 XH Amsterdam, Netherlands
关键词：
期刊名称：PROBIOTICS AND ANTIMICROBIAL PROTEINS （ 影响因子：4.4； 五年影响因子：5.0 ）
ISSN： 1867-1306
年卷期： 2025 年
页码：
收录情况： SCI
摘要： The biocontrol efficacy of probiotics Bacillus velezensis B31 against fusarium wilt is contingent upon its successful colonization in the plant rhizosphere. This study aimed to develop a quantitative detection method for probiotics strain B31 and to monitor its rhizosphere dynamics. Utilizing comparative genomics, a specific gene in strain B31 was identified, which facilitated the design of precise primers and a Taqman probe. The resulting real-time PCR assay demonstrated high specificity to strain B31 and high sensitivity with detection limit of 28 copies/mu L of the genome. Comparative analysis with regular PCR methods revealed a strong correlation, yet highlighted the superior sensitivity and rapidity of the real-time PCR technique. The temporal colonization profile of strain B31, post root-inoculation, was charted using this assay. An initial rhizosphere population of 7.2 x 107 CFU g-1 was observed, which then tapered to 105 CFU g-1 over 14 days. These dynamics corresponded with the observed control effect, where a higher initial population correlated with enhanced disease suppression. This study underscores the potential of real-time PCR as a robust tool for biocontrol agents' monitoring, offering insights into the microbial interactions within the rhizosphere.
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