Spent mushroom substrate amendment induces suppressiveness against cucumber Fusarium wilt through changes in the rhizosphere microbiome
文献类型： 外文期刊
作者： Qu, Yuanhang 1 ; Liu, Tiancong 1 ; Dong, Lihong 1 ; Dong, Lemeng 2 ; Su, Zhenhe 1 ; Bouwmeester, Harro 2 ; Guo, Qinggang 1 ; Ma, Ping 1 ;
作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Plant Protect,Minist Agr & Rural Affairs Chin, Integrated Pest Management Innovat Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China, Baoding 071000, Peoples R China
2.Univ Amsterdam, Swammerdam Inst Life Sci, Plant Hormone Biol Grp, Sci Pk 904, NL-1098 XH Amsterdam, Netherlands
关键词：
Cucumber
期刊名称：PLANT AND SOIL （ 影响因子：4.1； 五年影响因子：4.7 ）
ISSN： 0032-079X
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Background and aimsCucumber Fusarium wilt (CFW), caused by Fusarium oxysporum f. sp. cucumerinum (FOC), is a major soil-borne disease that limits cucumber production. In the present study the efficacy of spent mushroom substrate (SMS) amendment in suppressing CFW and the underlying microbial mechanisms are investigated.MethodsA pot experiment was conducted to assess the disease suppression and growth promotion effects of SMS amendment on cucumber. The rhizosphere microbiome composition, diversity, and functional potential were analyzed using 16S rRNA gene amplicon sequencing and shotgun metagenomic sequencing.ResultsThe application of 4% SMS effectively suppressed CFW incidence and reduced FOC abundance by tenfold compared to the control. A transplanting assay demonstrated the crucial role of the rhizosphere microbiome in SMS-mediated disease suppression and plant growth promotion. 16S rRNA sequencing revealed a significant enrichment of beneficial bacteria, particularly Bacillus spp., following SMS amendment. Functional analysis indicated that SMS amendment enhanced microbial pathways associated with dissimilatory nitrate reduction and glutamate synthesis, which are linked to the production of antimicrobial compounds. Furthermore, Bacillus velezensis SE58, isolated from SMS-amended rhizosphere, exhibited synergistic disease suppression when combined with SMS.ConclusionsSMS effectively suppresses CFW by selectively enriching beneficial rhizosphere bacteria, particularly Bacillus spp. These findings provide new insight into the protective effect of SMS and its potential application in the biological control of soil-borne plant disease.
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