Systematic Analysis of Dof Gene Family in Prunus persica Unveils Candidate Regulators for Enhancing Cold Tolerance
文献类型： 外文期刊
作者： Chen, Zheng 1 ; Wang, Xiaojun 1 ; Yan, Juan 1 ; Cai, Zhixiang 1 ; Zhang, Binbin 1 ; Xu, Jianlan 1 ; Ma, Ruijuan 1 ; Yu, Mingliang 1 ; Shen, Zhijun 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Pomol, Jiangsu Key Lab Hort Crop Genet Improvement, 50 Zhongling St, Nanjing 210014, Peoples R China
关键词：
5-aminolevulinic acid (ALA); cold stress;
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 15 期
页码：
收录情况： SCI
摘要： Late-spring frost events severely damage low-chill peach blossoms, causing significant yield losses. Although 5-aminolevulinic acid (ALA) enhances cold tolerance through the PpC3H37-PpWRKY18 module, the regulatory mechanism of ALA on PpC3H37 remains to be elucidated. Using yeast one-hybrid screening with the PpC3H37 promoter as bait, we identified PpDof9 as a key interacting transcription factor. A genome-wide analysis revealed 25 PpDof genes in peaches (Prunus persica). These genes exhibited variable physicochemical properties, with most proteins predicted as nuclear-localized. Subcellular localization experiments in tobacco revealed that PpDof9 was localized to the nucleus, consistent with predictions. A synteny analysis indicated nine segmental duplication pairs and tandem duplications on chromosomes 5 and 6, suggesting duplication events drove family expansion. A conserved motif analysis confirmed universal presence of the Dof domain (Motif 1). Promoter cis-element screening identified low-temperature responsive (LTR) elements in 12 PpDofs, including PpDof1, PpDof8, PpDof9, and PpDof25. The quantitative real-time PCR (qRT-PCR) results showed that PpDof1, PpDof8, PpDof9, PpDof15, PpDof16, and PpDof25 were significantly upregulated under low-temperature stress, and this upregulation was further enhanced by ALA pretreatment. Our findings demonstrate ALA-mediated modulation of specific PpDof TFs in cold response and provide candidates (PpDof1, PpDof9, PpDof8, PpDof25) for enhancing floral frost tolerance in peaches.
- 相关文献
作者其他论文 更多>>
-
Comparative transcriptomics of Monilinia fructicola- resistant and- susceptible peach fruit reveals gene networks associated with peach resistance to brown rot disease
作者：Li, Shenge;Xu, Jianlan;Cai, ZhiXiang;Ma, Ruijuan;Yu, Mingliang;Shen, Zhijun
关键词：Comparative transcriptome analysis; Disease resistance; NLR; Calcium signaling; Hypersensitive response
-
Assembly and analysis of the complete mitochondrial genome of Prunus davidiana (Rosaceae)
作者：Zhang, Yuyan;Ma, Ruijuan;Yu, Mingliang;Xu, Jianlan;Shen, Zhijun;Zhang, Yuyan;Guo, Xin
关键词：
Prunus davidiana ; mitochondrial genome; repeats; phylogenetic analysis; indel marker -
S-RNase-mediated attenuation of FviPP2Ac-FviRbohH binding promotes ROS accumulation and arrests pollen tube growth in Fragaria viridis
作者：Ni, Zhiyou;Zou, Yuting;Xu, Linlin;Shen, Zhijun;Wu, Ejiao;Qiao, Yushan;Ni, Zhiyou;Pan, Jiaoyan;Zou, Yuting;Pan, Zhiliang;Wang, Tao;Qiao, Yushan
关键词：NADPH oxidase; Phosphatase; ROS; S-RNase; Self-incompatibility; Strawberries
-
Graph pangenome reveals the regulation of malate content in blood-fleshed peach by NAC transcription factors
作者：Chen, Wenbo;Xie, Qi;Fu, Jia;Li, Shaojia;Shi, Yanna;Lu, Jiao;Zhao, Yingjie;Li, Baijun;Zhang, Bo;Chen, Kunsong;Chen, Wenbo;Li, Shaojia;Shi, Yanna;Zhang, Bo;Chen, Kunsong;Chen, Wenbo;Li, Shaojia;Shi, Yanna;Zhang, Bo;Grierson, Donald;Chen, Kunsong;Zhang, Yuanyuan;Ma, Ruijuan;Yu, Mingliang;Grierson, Donald;Fei, Zhangjun;Fei, Zhangjun
关键词：Graph pangenome; Peach; Malate content; Blood-fleshed; NAC
-
A deletion in a photosynthesis-related gene (PpPSAK) determines the anthocyanin accumulation in the mesocarp of French blood-flesh peaches
作者：Heurtevin, Laure;Lambert, Patrick;Pascal, Thierry;Quilot, Benedicte;Confolent, Carole;Bureau, Sylvie;Shen, Zhijun
关键词：Prunus; SNP mapping; Sequencing; RNAseq; qRT-PCR; PpMYB10 transcription factor
-
Ultrasound-Assisted Extraction Optimization and Cultivar Screening for Polyphenol Recovery from Thinned Peach Fruit: A Comprehensive Evaluation of 179 Cultivars
作者：Li, Shenge;Xu, Jianlan;Cai, Zhixiang;Guo, Shaolei;Yu, Mingliang;Shen, Zhijun
关键词：thinned peach; polyphenols; antioxidation; ultrasonic-assisted extraction; resources
-
The profiling of phytohormone levels with developmental progression in dormant floral buds in peach varieties of differing chilling requirements
作者：Yan, Juan;Li, Jiyao;Sun, Meng;Cai, Zhixiang;Zhang, Binbin;Xu, Jianlan;Ma, Ruijuan;Yu, Mingliang;Shen, Zhijun;Li, Jiyao
关键词：Chilling requirement; Dormancy; Jasmonic acid; Peach; Phytohormones
