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Development and molecular analysis of a novel acetohydroxyacid synthase rapeseed mutant with high resistance to sulfonylurea herbicides

文献类型： 外文期刊

作者： Guo, Yue 1 ; Liu, Changle 1 ; Long, Weihua 1 ; Gao, Jianqin 1 ; Zhang, Jiefu 1 ; Chen, Song 1 ; Pu, Huiming 1 ; Hu, Maolong 1 ;

作者机构： 1.Minist Agr, Inst Ind Crops, Key Lab Cotton & Rapeseed Nanjing, Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China

3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

4.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

关键词： Acetohydroxyacid synthase; High-resistance rapeseed; Additional round of mutagenesis; Sulfonylurea herbicide; Synergistic mutations

期刊名称：CROP JOURNAL （ 影响因子：4.647； 五年影响因子：5.781 ）

ISSN： 2095-5421

年卷期： 2022 年 10 卷 1 期

页码：

收录情况： SCI

摘要： With the increasing promotion of simplified rapeseed cultivation in recent years, the development of cultivars with high resistance to herbicides is urgently needed. We previously developed M342, which shows sulfonylurea herbicide resistance, by targeting acetohydroxyacid synthase (AHAS), a key enzyme in branched-chain amino acid synthesis. In the present study, we used a progeny line derived from M342 for an additional round of ethyl methane sulfonate mutagenesis, yielding the novel mutant DS3, which harbored two mutations in AHAS genes and showed high sulfonylurea resistance. One mutation was the substitution Trp574Leu, as in M342, according to Arabidopsis protein sequencing. The other site was a newly recognized substitution, Pro197Leu. A KASP marker targeting Pro197Leu was developed and reliably predicted the response to sulfonylurea herbicides in the F-2 population. The combination of Trp574Leu and Pro197Leu in DS3 produced a synergistic effect that greatly increased herbicide resistance. Analysis of the protein structures of AHAS1 and AHAS3 in wild-type and single-gene mutant plants revealed three-dimensional protein conformational changes that could account for differences in herbicide resistance characteristics including toxicity tolerance, AHAS enzyme activity, and AHAS gene expression. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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