Proteomic Analysis of Antinutritional Protein in Wild and Cultivated Soybean Seeds Based on Four-Dimensional Data-Independent Acquisition and Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging
文献类型： 外文期刊
作者： Ren, Zhentao 1 ; Liu, Laipan 1 ; Yin, Xin 1 ; Zhang, Li 1 ; Wang, Xiaojing 2 ; Shen, Wenjing 1 ; Fang, Zhixiang 1 ; Yu, Qi 1 ; Xue, Kun 3 ; Jia, Ruizong 4 ; Liu, Biao 1 ;
作者机构： 1.Nanjing Inst Environm Sci, Minist Ecol & Environm, Nanjing 210042, Peoples R China
2.Inner Mongolia Innovat Ctr Biol Breeding Technol, Hinggan League Inst Agr & Husb Sci, Ulan Hot 137400, Peoples R China
3.Minzu Univ China, Coll Life & Environm Sci, Ctr Imaging & Syst Biol, Beijing 100081, Peoples R China
4.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya 572011, Peoples R China
关键词：
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2025 年 73 卷 36 期
页码：
收录情况： SCI
摘要： Antinutritional proteins are the primary factors limiting the development of commercial varieties of cultivated soybeans from wild soybean. Four-dimensional data-independent acquisition and matrix-assisted laser desorption/ionization-mass spectrometry imaging were used for proteomic analysis of wild and cultivated soybean seeds. Fifteen antigenic proteins, 8 agglutinins, and 33 protease inhibitors were detected in the seeds. The antigenic proteins, such as Gly m 5, Gly m 6, and P34, were distributed primarily in the embryo and were more abundant in wild soybeans. No significant differences in the amount of agglutinin were observed between wild and cultivated soybeans. Protease inhibitors were primarily enriched in soybean embryos, and their presence in wild soybeans was higher than that in cultivated soybeans. The wild and cultivated soybean embryos showed slightly different distributions of antigenic proteins and protease inhibitors, which were distributed primarily in the hypocotyls and cotyledons. These results can be used to assess the benefits and risks of high antinutritional protein expression.
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