Csn5 inhibits autophagy by regulating the ubiquitination of Atg6 and Tor to mediate the pathogenicity of Magnaporthe oryzae
文献类型： 外文期刊
作者： Shen, Zi-Fang 1 ; Li, Lin 1 ; Wang, Jing-Yi 2 ; Liao, Jian 2 ; Zhang, Yun-Ran 2 ; Zhu, Xue-Ming 1 ; Wang, Zi-He 2 ; Lu, Jian-Ping 3 ; Liu, Xiao-Hong 2 ; Lin, Fu-Cheng 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Treats Qual & S, Zhejiang Prov Key Lab Agr Microbi, Key Lab Agr Microbiome MARA,Inst Plant Protect & M, Hangzhou 310021, Peoples R China
2.Zhejiang Univ, State Key Lab Managing Biot & Chem Treats Qual & S, Zhejiang Prov Key Lab Agr Microbi, Key Lab Agr Microbiome MARA,Inst Biotechnol, Hangzhou 310058, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China
关键词： COP9 signalosome; Csn5; Autophagy; Ubiquitination; Pathogenicity; Rice blast fungus
期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.4； 五年影响因子：8.0 ）
ISSN：
年卷期： 2024 年 22 卷 1 期
页码：
收录情况： SCI
摘要： Csn5 is subunit 5 of the COP9 signalosome (CSN), but the mechanism by which it strictly controls the pathogenicity of pathogenic fungi through autophagy remains unclear. Here, we found that Csn5 deficiency attenuated pathogenicity and enhanced autophagy in Magnaporthe oryzae. MoCSN5 knockout led to overubiquitination and overdegradation of MoTor (the core protein of the TORC1 complex [target of rapamycin]) thereby promoted autophagy. In addition, we identified MoCsn5 as a new interactor of MoAtg6. Atg6 was found to be ubiquitinated through linkage with lysine 48 (K48) in cells, which is necessary for infection-associated autophagy in pathogenic fungi. K48-ubiquitination of Atg6 enhanced its degradation and thereby inhibited autophagic activity. Our experimental results indicated that MoCsn5 promoted K48-ubiquitination of MoAtg6, which reduced the MoAtg6 protein content and thus inhibited autophagy. Aberrant ubiquitination and autophagy in Delta Mocsn5 led to pleiotropic defects in the growth, development, stress resistance, and pathogenicity of M. oryzae. In summary, our study revealed a novel mechanism by which Csn5 regulates autophagy and pathogenicity in rice blast fungus through ubiquitination.
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