文献类型： 外文期刊

作者： Hu Fu-Chu ¹ ; Chen Zhe ² ; Wang Xiang-He ² ; Wang Jia-Bao ³ ; Fan Hong-Yan ² ; Qin Yong-Hua ¹ ; Zhao Jie-Tang ¹ ; Hu Gui- ¹ ;

作者机构： 1.South China Agr Univ, Minist Agr & Rural Affairs, State Key Lab Conservat & Utilizat Subtrop Agrobi, Coll Hort,Key Lab Biol & Genet Improvement Hortic, Guangzhou 510642, Peoples R China

2.Hainan Acad Agr Sci, Inst Trop Fruit Trees, Key Lab Trop Fruit Tree Biol Hainan Prov, Haikou 571100, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

关键词： Litchi chinensis; SNP genetic map; dwarf-related traits; QTL analysis; genotyping-by-sequencing (GBS)

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2021 年 20 卷 11 期

页码：

收录情况： SCI

摘要： Litchi chinensis Sonn is widely cultivated in subtropical regions and has an important economic value. A high-density genetic map is a valuable tool for mapping quantitative trait loci (QTL) and marker-assisted breeding programs. In this study, a single nucleotide polymorphism (SNP)-based high-density linkage map was constructed by a genotyping-by-sequencing (GBS) protocol using an F1 population of 178 progenies between two commercial litchi cultivars, 'Ziniangxi' (dwarf) and 'Feizixiao' (vigorous). The genetic map consisted of 3 027 SNP markers with a total length of 1 711.97 cM in 15 linkage groups (LGs) and an average marker distance of 0.57 cM. Based on this high-density linkage map and three years of phenotyping, a total of 37 QTLs were detected for eight dwarf-related traits, including length of new branch (LNB), diameter of new branch (DNB), length of common petiole (LCP), diameter of common petiole (DCP), length of internode (LI), length of single leaf (LSL), width of single leaf (WSL), and plant height (PH). These QTLs could explain 8.0 to 14.7% (mean=9.7%) of the phenotypic variation. Among them, several QTL clusters were observed, particularly on LG04 and LG11, which might show enrichment for genes regulating the dwarf-related traits in litchi. There were 126 candidate genes identified within the QTL regions, 55 of which are differentially expressed genes by RNA-seq analysis between 'Ziniangxi' and 'Feizixiao'. These DEGs were found to participate in the regulation of cell development, material transportation, signal transduction, and plant morphogenesis, so they might play important roles in regulating plant dwarf-related traits. The high-density genetic map and QTLs identification related to dwarf traits can provide a valuable genetic resource and a basis for marker-assisted selection and genomic studies of litchi.