文献类型： 外文期刊

作者： Wei, Yidong ¹ ; Xie, Hongguang ¹ ; Diao, Lirong ¹ ; Wang, Jinlan ¹ ; Wu, Fangxi ¹ ; Luo, Xi ¹ ; He, Wei ¹ ; Pan, Liyan ¹ ; Xie, Huaan ¹ ; Zhang, Jianfu ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Rice Res Inst, Fuzhou 350019, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Germplasm Innovat & Mol Breeding Hybrid R, Natl Rice Improvement Ctr China,Fuzhou Branch,Sta, Incubator Natl Key Lab Germplasm Innovat & Mol Br, Fuzhou 350003, Peoples R China

3.Natl Rice Engn Lab, Fuzhou 350003, Peoples R China

关键词： rice; protein L-isoaspartyl methyltransferase (PIMT); two-dimensional electrophoresis; seed vigor; different expression

期刊名称：CHINESE SCIENCE BULLETIN-CHINESE （ 影响因子：1.1； ）

ISSN： 0023-074X

年卷期： 2020 年 65 卷 2-3 期

页码：

收录情况： SCI

摘要： China is the largest rice producing and consuming country in the world. As the main food crop in China, the stable production of rice is of great significance to food security. However, in the process of rice storage, due to aging and mildew problems, billions of kilograms of rice are lost every year, accounting for about 3% of the total reserves of rice, which not only affects food security, but also poses a great threat to food security. With the development of molecular biology technology, researching and solving the problem of rice storage tolerance from the perspective of biochemistry and molecular biology technology will provide technical support and guarantee for the development and breeding of new rice varieties with storage tolerance. Seed aging during storage is the result of various cellular functions, including changes in enzyme activity, cell membrane damage, DNA and stored RNA damage, fat and sugar metabolism, etc. It is believed that spontaneous covalent damage of proteins in cells is the fundamental cause of aging. Aging can be seen as a battle between the internal protection system of organisms and its accumulated damage during aging. In general, damaged proteins can be repaired by re-synthesis. However, in some cases, organisms need to repair damaged proteins without protein synthesis. The seeds of plants are in a condition of low metabolism and low material supply, which means that protein synthesis almost stops. To maintain a relatively stable state under aging conditions, plant seeds must use a low-resource and energy-depletion approach to repair damage, without requiring protein synthesis. Spontaneous formation of isoaspartyl (isoAsp) residues from aspartic acid and asparagine in proteins are common covalent damage of proteins found in cells. Based on previous study, PIMT1-related seed longevity improvement is probably due to the repair of detrimental isoAsp-containing proteins that over accumulate in embryos when subjected to accelerated aging. However, the mechanism of Protein L-isoaspartyl methyltransferase (PIMT) on rice seed vigor is still unclear. In this study, OsPIMT1 underexpression materials were generated by RNA interference, and the result of artificial aging shows that low expression of OsPIMT1 reduced seed germination vigor. The short-time germination percentage of OsPIMT1 RNAi transgenic seeds declined to less than 70% after 5-day of artificial aging, which indicated that seed vigor of OsPIMT1 RNAi was impaired even at the early stage of artificial aging. Furthermore, dynamic changes of isoaspartyl containing proteins in embryo were detected, which indicated that OsPIMT1 expression reduced the accumulation of isoAsp in both non-imbibed and imbibed seeds and the seed vigor loss was probably caused by overaccumulation of isoAsp-containing proteins in RNAi lines of OsPIMT1 inhibition. At the same time, embryo proteins of 5-day-aging seeds were separated using two-dimensional electrophoresis to analyze the effect of overaccumulation of isoAsp-containing proteins in RNAi materials during aging. The proteins related to seed aging were identified, including the oxidative stress-related proteins, enzymes of glycolysis, and heat shock proteins. Geneontology and KEGG analysis were performed to enrich biological process and pathway of differential proteins. These results laid the foundation for further study of the role of OsPIMT1 in the metabolic pathways of cells after seed aging.