文献类型： 外文期刊

作者： Liu, Shiqing ¹ ; Wang, Jiancheng ² ; Li, Xuerong ² ; Wang, Xianhang ⁵ ; Wang, Xiyong ² ; Zhong, Haixia ⁶ ; Aibibul, Ilham ⁷ ; Sun, Feng ⁷ ; Li, Hongbin ¹ ; Shi, Wei ¹ ;

作者机构： 1.Shihezi Univ, Coll Life Sci, Shihezi 832003, Peoples R China

2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Key Lab Ecol Safety & Sustainable Dev Arid Lands, Urumqi 830011, Peoples R China

3.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Xinjiang Key Lab Conservat & Utilizat Plant Gene R, Urumqi 830011, Peoples R China

4.Chinese Acad Sci, Turpan Eremophytes Bot Garden, Turpan 838008, Peoples R China

5.Northwest A&F Univ, Viti Viniculture Engn Technol Ctr State Forestry &, Shaanxi Engn Res Ctr Viti Viniculture, Coll Enol,Coll Food Sci & Engn,Heyang Viti Vinicul, Yangling 712100, Peoples R China

6.Xinjiang Acad Agr Sci, Inst Hort Crops, Urumqi 830091, Peoples R China

7.Peoples Govt Lianmuqin Town, Shanshan 838200, Peoples R China

关键词： Thompson Seedless; Munake grapes; whole-genome resequencing; genetic diversity; SSR; SNP; germplasm resource safety; intensive cultivation

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 15 卷 8 期

页码：

收录情况： SCI

摘要： This study systematically investigated the genetic characteristics and germplasm conservation strategies of Xinjiang Thompson Seedless grapes (Vitis vinifera Thompson Seedless) and Munake grapes (Vitis vinifera L. cv. Munake) using SSR molecular markers and whole-genome resequencing technology. A genetic diversity analysis of 165 Thompson Seedless accessions with 16 SSR markers identified 442 alleles (27.63 alleles per locus on average), with the expected heterozygosity (He = 0.76) and observed heterozygosity (Ho = 0.83) indicating moderate-to-low genetic diversity. A molecular variance analysis (AMOVA) further revealed that 96% of the genetic variation originated within populations, with minimal inter-population differentiation (Fst = 0.04). Phylogenetic reconstruction using SSR markers demonstrated significant genealogical associations between the two cultivars, supporting the hypothesis that Thompson Seedless may have originated from a domesticated lineage of Munake. The selected SSR markers exhibited high discriminatory power (PIC = 0.92), enabling the precise differentiation of accessions with closely related genetic backgrounds. Whole-genome resequencing identified 20,074,046 and 69,214,080 high-quality SNPs in 100 Thompson Seedless and 141 Munake accessions, respectively. Core germplasm banks were subsequently established: the Thompson Seedless core collection (25 accessions) captured 94% of genetic variation, reflecting genetic homogenization driven by intensive clonal cultivation, while the Munake core collection (42 accessions) retained 95% of allelic diversity and resolved regional synonym issues through highly polymorphic SNP markers. A comparative analysis revealed that Munake maintains higher genetic diversity due to natural gene flow, whereas Thompson Seedless faces heightened risks of genetic erosion from prolonged asexual propagation. These findings provide a theoretical foundation and technical framework for precise conservation, varietal improvement, and sustainable utilization of grape germplasm resources in Xinjiang.