Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates
文献类型： 外文期刊
作者： Zhang, Yan 1 ; Qiu, Yu 1 ; Liu, Kai 4 ; Zhong, Wenjun 1 ; Yang, Jianghua 1 ; Altermatt, Florian 2 ; Zhang, Xiaowei 1 ;
作者机构： 1.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Peoples R China
2.Univ Zurich, Dept Evolutionary Biol & Environm Studies, Zurich, Switzerland
3.Eawag Swiss Fed Inst Aquat Sci & Technol, Dept Aquat Ecol, Dubendorf, Switzerland
4.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr & Rural Affairs, Wuxi 214081, Peoples R China
关键词： Environmental nucleic acids; Aquatic biodiversity; Organismal size; Species detectability
期刊名称：ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY （ 影响因子：14.0； 五年影响因子：13.2 ）
ISSN： 2666-4984
年卷期： 2024 年 21 卷
页码：
收录情况： SCI
摘要： The monitoring and management of aquatic ecosystems depend on precise estimates of biodiversity. Metabarcoding analyses of environmental nucleic acids (eNAs), including environmental DNA (eDNA) and environmental RNA (eRNA), have garnered attention for their cost-effective and non-invasive biomonitoring capabilities. However, the accuracy of biodiversity estimates obtained through eNAs can vary among different organismal groups. Here we evaluate the performance of eDNA and eRNA metabarcoding across nine organismal groups, ranging from bacteria to terrestrial vertebrates, in three crosssections of the Yangtze River, China. We observe robust complementarity between eDNA and eRNA data. The relative detectability of eNAs was notably influenced by major taxonomic groups and organismal sizes, with eDNA providing more robust signals for larger organisms. Both eDNA and eRNA exhibited similar cross-sectional and longitudinal patterns. However, the detectability of larger organisms declined in eRNA metabarcoding, possibly due to differential RNA release and decay among different organismal groups or sizes. While underscoring the potential of eDNA and eRNA in large river biomonitoring, we emphasize the need for differential interpretation of eDNA versus eRNA data. This highlights the importance of careful method selection and interpretation in biomonitoring studies. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Chinese Society for Environmental Sciences, Harbin Institute of Technology, Chinese Research Academy of Environmental Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- 相关文献
作者其他论文 更多>>
-
Estimates on age, growth, and mortality of Leuciscus chuanchicus (Kessler 1876) in the Ningxia section of the upper reaches of the Yellow River, China
作者：Xiong, Shuhan;Liu, Jiacheng;Li, Peilun;Wang, Jilong;Xiong, Shuhan;Liu, Jiacheng;Li, Peilun;Wang, Jilong;Xiong, Shuhan;Liu, Jiacheng;Li, Peilun;Wang, Jilong;Liu, Yanbin;Liu, Kai;Wang, Yongjie
关键词：Leuciscus chuanchicus; Growth characteristics; Mortality estimates; Exploitation rate
-
Generation of transgenic fish cell line with a-lactalbumin nanocarriers co-delivering Tol2 transposase mRNA and plasmids
作者：Zhao, Ran;Zhang, Yan;Wang, Qi;Cao, Yi-Ming;Hou, Ming-Xi;Sun, Xiao-Qing;Yu, Shuang-Ting;Li, Jiong-Tang;Zhao, Ran;Zhang, Yan;Wang, Qi;Cao, Yi-Ming;Hou, Ming-Xi;Sun, Xiao-Qing;Yu, Shuang-Ting;Li, Jiong-Tang;Yu, Shuang-Ting;Chen, Ying-Jie;Wang, Kai-Kuo
关键词：
-
High risk of biological invasion from prayer animal release in China
作者：Du, Yuanbao;Xi, Yonghong;Tu, Weishan;Cui, Ruina;Yan, Zhuo;Liu, Xuan;Xi, Yonghong;Liu, Xuan;Yang, Zhixu;Gu, Dangen;Zhang, Zhixin;Zhang, Zhixin;Tu, Weishan;Guo, Baocheng;Tu, Weishan;Zeng, Yan;Xin, Yusi;Jin, Wenjia;Zhang, Yan;Yang, Le;Ke, Zunwei;Rohr, Jason R.
关键词：
-
A comparative study on the age, growth, and mortality of Gobio huanghensis (Luo, Le & Chen, 1977) in the Gansu and Ningxia sections of the upper Yellow River, China
作者：Li, Peilun;Liu, Jiacheng;Wang, Jilong;Li, Peilun;Wang, Jilong;Liu, Jiacheng;Liu, Yanbin;Liu, Kai;Wang, Tai
关键词：G. Huanghensis; Age; Growth; Mortality; Exploitation rate; Yellow River
-
Vocalization Pattern and Echolocation Signal Characteristics of Yangtze Finless Porpoise (Neophocaena asiaeorientalis asiaeorientalis) in Captivity
作者：Chen, Jia;Liang, Haiying;Liu, Kai;Lin, Danqing;Zhang, Jialu;Li, Dong;Ye, Kun;Lu, Wenfei;Liu, Kai
关键词：Yangtze finless porpoise; echolocation signal; behavioral state; diurnal variation; ambiguity function
-
Characterization of the Ovarian Development and Associated Factors during the Breeding Migration of Coilia nasus in the Yangtze River
作者：Wei, Shuwei;Ma, Fengjiao;Ying, Congping;Liu, Kai;Hua, Zhong;Yang, Yanping;Zhang, Wei;Deng, Yanmin;Liu, Kai;Han, Wei
关键词：Coilia nasus; ovarian development; organizational observation; sex steroid hormones; gene expression
-
Ascaridoid Nematodes Infection in Anadromous Fish Coilia nasus from Yangtze River
作者：Zhou, Qingjie;Wang, Lijun;Xi, Bingwen;Ying, Congping;Liu, Kai;Zhou, Qingjie;Wang, Lijun;Xi, Bingwen;Ying, Congping;Liu, Kai
关键词：Coilia nasus; parasite; nematode; Anisakis
