Unraveling the Genetic Architecture of Two Complex, Stomata-Related Drought-Responsive Traits by High-Throughput Physiological Phenotyping and GWAS in Cowpea (Vigna. Unguiculata L. Walp)
文献类型： 外文期刊
作者： Wu, Xinyi 1 ; Sun, Ting 2 ; Xu, Wenzhao 3 ; Sun, Yudong 3 ; Wang, Baogen 1 ; Wang, Ying 1 ; Li, Yanwei 1 ; Wang, Jian 1 ; Wu 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Peoples R China
2.China Jiliang Univ, Coll Life Sci, Hangzhou, Peoples R China
3.Huaiyin Inst Agr Sci Xuhuai Reg Jiangsu, Huaian, Peoples R China
4.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
关键词： GWAS; phenomic; drought; cowpea; stomatal
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： Drought is one of the most devasting and frequent abiotic stresses in agriculture. While many morphological, biochemical and physiological indicators are being used to quantify plant drought responses, stomatal control, and hence the transpiration and photosynthesis regulation through it, is of particular importance in marking the plant capacity of balancing stress response and yield. Due to the difficulties in simultaneous, large-scale measurement of stomatal traits such as sensitivity and speed of stomatal closure under progressive soil drought, forward genetic mapping of these important behaviors has long been unavailable. The recent emerging phenomic technologies offer solutions to identify the water relations of whole plant and assay the stomatal regulation in a dynamic process at the population level. Here, we report high-throughput physiological phenotyping of water relations of 106 cowpea accessions under progressive drought stress, which, in combination of genome-wide association study (GWAS), enables genetic mapping of the complex, stomata-related drought responsive traits "critical soil water content" (theta(cri)) and "slope of transpiration rate declining" (K-Tr). The 106 accessions showed large variations in theta(cri) and K-Tr, indicating that they had broad spectrum of stomatal control in response to soil water deficit, which may confer them different levels of drought tolerance. Univariate GWAS identified six and fourteen significant SNPs associated with theta(cri) and K-Tr, respectively. The detected SNPs distributed in nine chromosomes and accounted for 8.7-21% of the phenotypic variation, suggesting that both stomatal sensitivity to soil drought and the speed of stomatal closure to completion were controlled by multiple genes with moderate effects. Multivariate GWAS detected ten more significant SNPs in addition to confirming eight of the twenty SNPs as detected by univariate GWAS. Integrated, a final set of 30 significant SNPs associated with stomatal closure were reported. Taken together, our work, by combining phenomics and genetics, enables forward genetic mapping of the genetic architecture of stomatal traits related to drought tolerance, which not only provides a basis for molecular breeding of drought resistant cultivars of cowpea, but offers a new methodology to explore the genetic determinants of water budgeting in crops under stressful conditions in the phenomics era.
- 相关文献
作者其他论文 更多>>
-
Green synthesis of silver nanoparticles using Houttuynia cordata Thunb rhizome extracts and their antibacterial potential against common foodborne pathogens
作者：Maimaiti, Xiayidan;Liu, Xiaoli;Rahman, Nurgul;Luo, Runmeng;Maimaiti, Xiayidan;Bassey, Anthony Pius;Liu, Xiaoli;Zhu, Yongsheng;Fan, Linlin;Luo, Runmeng;Wang, Fan;Wang, Ying
关键词：Antibacterial activity; green synthesis; Houttuynia cordata; particle size; silver nanoparticles
-
Yield, Quality, and Nitrogen Leaching of Open-Field Tomato in Response to Different Nitrogen Application Measures in Northwestern China
作者：Mao, Xinping;Wang, Kun;Han, Fengpeng;Mao, Xinping;Wang, Fang;Liu, Ruliang;Hong, Yu;Wang, Ying;Mao, Xinping;Wang, Fang;Liu, Ruliang;Hong, Yu;Gu, Jialin;Han, Fengpeng
关键词：agricultural non-point source pollution; nitrogen use efficiency; nitrogen leaching; open-field tomato; principal component analysis; quality
-
Effect of metal ions on haze formation in blackberry wine
作者：Wu, Gang;Liu, Xiaoli;Zhou, Jianzhong;Wang, Ying;Wu, Gang;Wu, Caie;Wu, Shuang
关键词：Blackberry wine; Haze; Protein; Polyphenol; Metal ion
-
Micro-nano bubble water with potassium fertigation improves strawberry yield and quality by changing soil bacterial community
作者：Wang, Jian;He, Qiu;Wang, Duo;Chen, Rui;Zheng, Zhen;Cao, Kai;Zhou, Bo;Niu, Xiaoli
关键词：Micro -nano bubble water drip irrigation; Reduced K fertilization; Soil respiration; Strawberry quality; Yield; Soil bacterial community
-
Exogenous abscisic acid improves grain filling capacity under heat stress by enhancing antioxidative defense capability in rice
作者：Liu, Xiaolong;Zhong, Xin;Liao, Jingpeng;Ji, Ping;Yang, Jinshuo;Cao, Zhiruo;Duan, Ximiao;Xiong, Junru;Wang, Ying;Yang, Hongtao;Peng, Bo;Jiang, Kai;Liu, Xiaolong;Yang, Hongtao;Peng, Bo;Jiang, Kai;Xu, Chen
关键词：Abscisic acid (ABA); Heat stress; Priming Rice (Oryza sativa L.); Grain filling
-
Metal-induced color change in blackberry wine
作者：Wu, Gang;Wu, Caie;Wu, Gang;Fan, Linlin;Zhou, Jianzhong;Liu, Xiaoli;Wu, Caie;Wang, Ying;Wu, Shuang
关键词：Blackberry wine; Chelate; Iron; Color; Intermolecular interaction
-
Apigenin suppresses mycoplasma-induced alveolar macrophages necroptosis via enhancing the methylation of TNF-alpha promoter by PPAR gamma-Uhrf1 axis
作者：Mei, Xiuzhen;Feng, Zhixin;Zhang, Zhenzhen;Mei, Xiuzhen;Wang, Jian;Liu, Beibei;Xie, Qingyun;Yuan, Ting;Wu, Yuzi;Chen, Rong;Xie, Xing;Wei, Yanna;Wang, Li;Shao, Guoqing;Xiong, Qiyan;Feng, Zhixin;Zhang, Zhenzhen;Zhang, Chao;Zhu, Jiale;Zhu, Jiale;Xu, Yefen;Feng, Zhixin;Xu, Yefen;Feng, Zhixin;Zhang, Zhenzhen
关键词：Apigenin; Mycoplasma pneumonia; Necroptosis; Alveolar macrophages
