您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Magnaporthe oryzae endoplasmic reticulum membrane complex regulates the biogenesis of membrane proteins for pathogenicity

文献类型： 外文期刊

作者： Liu, Ning 1 ; Huang, Manna 1 ; Liang, Xinyuan 1 ; Cao, Miao 1 ; Lun, Zhiqin 1 ; Zhang, Yan 1 ; Yang, Jun 1 ; Bhadauria, Vijai 1 ; Zhao, Wensheng 1 ; Yan, Jiye 3 ; Peng, You-Liang 1 ; Lu, Xunli 1 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Fruit, Beijing 100097, Peoples R China

关键词： Atg9; cell wall integrity; Chs4; Fks1; Magnaporthe oryzae; magnesium transport; Mnr2; MoEmc5

期刊名称：NEW PHYTOLOGIST （ 影响因子：9.4； 五年影响因子：10.5 ）

ISSN： 0028-646X

年卷期： 2023 年 238 卷 3 期

页码：

收录情况： SCI

摘要： center dot In eukaryotes, the majority of newly synthesized integral membrane proteins are inserted into the endoplasmic reticulum (ER) membrane before transferred to their functional sites. The conserved ER membrane complex (EMC) takes part in the insertion process for tail anchored membrane proteins. However, the function of EMC in phytopathogenic fungi has not been characterized.center dot Here, we report the identification and functional characterization of two EMC subunits MoEmc5 and MoEmc2 in Magnaporthe oryzae. The knockout mutants AMoemc5 and delta Moemc2 exhibit substantial defect in autophagy, pathogenicity, cell wall integrity, and magnesium ion sensitivity.center dot We demonstrate that the autophagy process was severely impaired in the AMoemc5 and delta Moemc2 mutants because of the low-protein steady-state level of Atg9, the sole membrane-associated autophagy protein. Furthermore, the protein level of membrane proteins Chs4, Fks1, and MoMnr2 is also significantly reduced in the delta Moemc5 and delta Moemc2 strains, leading to their supersensitivity to Calcofluor white, Congo red, and magnesium. In addition, MoEmc5, but not MoEmc2, acts as a magnesium transporter independent of its EMC function.center dot Magnaporthe oryzae EMC regulates the biogenesis of membrane proteins for autophagy and virulence; therefore, EMC subunits could be potential targets for fungicide design in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台