Fluorescent and Colorimetric Dual-Readout Immunochromatographic Assay for the Detection of Phenamacril Residues in Agricultural Products
文献类型： 外文期刊
作者： Liu, Pengyan 1 ; Jiang, Lan 1 ; Zhao, Yun 5 ; Wang, Yulong 1 ; Ye, Yuhui 1 ; Xue, Feng 6 ; Hammock, Bruce D. 7 ; Zhang, Cunzheng 1 ;
作者机构： 1.Minist Sci & Technol, Jiangsu Key Lab Food Qual, Safety State Key Lab Cultivat Base, Nanjing 210014, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing 210014, Peoples R China
3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Nanjing 210014, Peoples R China
4.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China
5.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
6.Nanjing Agr Univ, Joint Int Res Lab Anim Hlth & Food Safety, Minist Educ, Nanjing 210095, Peoples R China
7.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
8.Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： fluorescent and colorimetric dual-readout; immunochromatographicassay; phenamacril; monoclonal antibody; polydopamine coated gold nanoparticles; quantum dots
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 19 期
页码：
收录情况： SCI
摘要： The fungicide phenamacril has been employed to manage Fusarium and mycotoxins in crops, leading to persistent residues in the environment and plants. Detecting phenamacril is pivotal for ensuring environmental and food safety. In this study, haptens and artificial antigens were synthesized to produce antiphenamacril monoclonal antibodies (mAbs). Additionally, gold nanoparticles coated with a polydopamine shell were synthesized and conjugated with mAbs, inducing fluorescence quenching in quantum dots. Moreover, a dual-readout immunochromatographic assay that combines the positive signal from fluorescence with the negative signal from colorimetry was developed to enable sensitive and precise detection of phenamacril within 10 min, achieving detection limits of 5 ng/mL. The method's reliability was affirmed by using spiked wheat flour samples, achieving a limit of quantitation of 0.05 mg/kg. This analytical platform demonstrates high sensitivity, outstanding accuracy, and robust tolerance to matrix effects, making it suitable for the rapid, onsite, quantitative screening of phenamacril residues.
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