Agave macroacantha Transcriptome Reveals Candidate CNGC Genes Responsive to Cold Stress in Agave
文献类型： 外文期刊
作者： Li, Yubo 1 ; Hu, Xiaoli 2 ; Mkapa, Dietram Samson 2 ; Xie, Li 5 ; Guo, Pingan 6 ; Tan, Shibei 2 ; Zhang, Weiyi 1 ; Chen, Helong 2 ; Huang, Xing 2 ; Yi, Kexian 9 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Danzhou 571737, Peoples R China
2.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Natl Key Lab Trop Crop Breeding, Haikou 571101, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
4.Tanzania Agr Res Inst TARI, Mlingano Ctr, POB 5088, Tanga, Tanzania
5.Pengshui Miao Tujia Autonomous Cty Chongqing Agr &, Chongqing 409600, Peoples R China
6.Hubei Normal Univ, Hubei Key Lab Edible Wild Plants Conservat & Utili, Huangshi 435002, Peoples R China
7.Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Peoples R China
8.Hainan Key Lab Monitoring & Control Trop Agr Pests, Haikou 571101, Peoples R China
9.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Peoples R China
关键词：
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Agave, with its unique appearance and ability to produce hard fibers, holds high economic value. However, low temperatures during winter can restrict its growth and even damage the leaves, causing a loss of ornamental appeal or affecting the fiber quality. Conversely, the plant cyclic nucleotide-gated channel (CNGC) family plays an important role in the growth and development of plants and the response to stress. Studying the CNGC family genes is of great importance for analyzing the mechanism by which agave responds to cold stress. This research conducted a transcriptomic analysis of the ornamental plant Agave macroacantha. Through assembly via Illumina sequencing, 119,911 transcripts were obtained, including 78,083 unigenes. In total, 6, 10, 11, and 13 CNGC genes were successfully identified from A. macroacantha, Agave. H11648, Agave. deserti, and Agave. tequilana, respectively. These CNGC genes could be divided into four groups (I, II, III, and IV), and group IV could be divided into two subgroups (IV-A and IV-B). The relative expression levels were quantified by qRT-PCR assays, which revealed that AhCNGC4.1 was significantly upregulated after cold treatment and Ca(NO3)2 treatment, suggesting its importance in cold stress and calcium signaling. Additionally, the Y2H assay has preliminarily identified interacting proteins of AhCNGC4.1, including AhCML19 and AhCBSX3. This study has established a completely new transcriptome dataset of A. macroacantha for the first time, enriching the bioinformatics of agave's transcriptome. The identified CNGC genes are of great significance for understanding the evolution of agave species. The cloned CNGC genes, expression pattern analysis, and protein interaction results laid a foundation for future research related to the molecular functions of agave CNGC genes in cold tolerance.
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