Genome wide analysis of HMA gene family in Hydrangea macrophylla and characterization of HmHMA2 in response to aluminum stress
文献类型： 外文期刊
作者： Ahmad, Muhammad Zulfiqar 1 ; Chen, Shuangshuang 1 ; Qi, Xiangyu 1 ; Feng, Jing 1 ; Chen, Huijie 1 ; Liu, Xintong 1 ; Sun, Ming 2 ; Deng, Yanming 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Leisure Agr, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China
2.Beijing Forestry Univ, State Key Lab Efficient Prod Forest Resources, Beijing 100083, Peoples R China
关键词： Hydrangea macrophylla; HMA gene family; Aluminum stress; HMA2; Gene expression profile; Abiotic stress
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）
ISSN： 0981-9428
年卷期： 2024 年 216 卷
页码：
收录情况： SCI
摘要： Aluminum toxicity poses a significant threat to plant growth, especially in acidic soils. Heavy metal ATPases (HMAs) are crucial for transporting heavy metal ions across plant cell membranes, yet their role in Al3+ transport remains unexplored. This study identified eight HmHMA genes in the genome of Hydrangea macrophylla, categorizing them into two major clades based on phylogenetic relationships. These genes were found unevenly distributed across six chromosomes. Detailed analysis of their physicochemical properties, collinearity, and gene structure was conducted. RNA-seq and qRT-PCR analyses revealed that specific HmHMA genes, notably HmHMA2, were predominantly expressed in roots and flowers under Al3+ stress, indicating their potential role in Al3+ tolerance. HmHMA2 showed significant expression in roots, especially under Al3+ stress conditions, and when expressed in yeast cells, it conferred resistance to aluminum and zinc but increased sensitivity to cadmium. Overexpression of HmHMA2 in hydrangea leaf discs significantly improved Al3+ tolerance, reduced oxidative stress markers like hydrogen peroxide and malondialdehyde, and enhanced antioxidant enzyme activity such as SOD, POD and CAT compared to controls. These findings shed lights on the potential role of HmHMAs in Al transport and tolerance in H. macrophylla.
- 相关文献
作者其他论文 更多>>
-
Genome wide and evolutionary analysis of ALOG gene family and its role during seed development and nodulation in response to rhizobium in soybean
作者：Ahmad, Muhammad Zulfiqar;Ahmad, Bushra;Nasir, Jamal Abdul;Jamil, Arshad;Ali, Saqib;Gul, Asma;Alarfaj, Abdullah A.;Alharbi, Sulaiman Ali
关键词：Soybean; ALOG gene family; Genome wide analysis; Evolutionary analysis; Synteny analysis
-
The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem
作者：Aljedaani, Fatimah;Luo, Yinghui;Smet, Wouter;Xu, Xinjing;Xiao, Ting Ting;Gonzalez-Kise, Jose Kenyi;Blilou, Ikram;Deng, Yanming;Nasim, Zeeshan;Hameed, Umar F. Shahul;Arold, Stefan
关键词：
-
Integrated Analysis of Metatranscriptome and Amplicon Sequencing to Reveal Distinctive Rhizospheric Microorganisms of Salt-Tolerant Rice
作者：Meng, Wenna;Tan, Mingpu;Liu, Anqi;Liu, Shuai;Wang, Jiaxue;Deng, Yanming;Zhou, Zhenling;Sun, Zhiguang;Tan, Yiluo;Liu, Yan;Wang, Baoxiang;Deng, Yanming
关键词：metatranscriptome; plant-microbe interaction; salt tolerance; rice
-
Overexpression of ATP binding cassette transporters (ABCs) from Hydrangea macrophylla enhance aluminum tolerance
作者：Chen, Shuangshuang;Chen, Wenjuan;Liu, Yiming;Ahmad, Muhammad Zulfiqar;Feng, Jing;Chen, Huijie;Qi, Xiangyu;Deng, Yanming;Chen, Wenjuan;Deng, Yanming;Liu, Yiming;Deng, Yanming
关键词：Hydrangea macrophylla; ATP-binding cassette (ABC) transporters; Aluminum accumulation; 3D structure; Surface pockets
-
Identification of Nramp gene family in Hydrangea macrophylla and characterization of HmNramp5 under aluminum stress condition
作者：Ahmad, Muhammad Zulfiqar;Chen, Shuangshuang;Qi, Xiangyu;Feng, Jing;Chen, Huijie;Liu, Xintong;Deng, Yanming;Deng, Yanming
关键词：Aluminum tolerance; Hydrangea; HmNramp5; Acidic soil; Aluminum toxicity
-
A genome-wide approach to the comprehensive analysis of the serine carboxypeptidase-like protein family in Solanum lysopersicum L.
作者：Jamil, Arshad;Habib, M. A.;Ibrahim, Ahmed B. M.;Ahmad, Bushra;Ahmad, Muhammad Zulfiqar;Nasir, Jamal Abdul
关键词：Serine carboxypeptidases-like protein; Genome-wide analysis; Drought stress; Heat stress; Tomato
-
The chromosome-level genome of double-petal phenotype jasmine provides insights into the biosynthesis of floral scent
作者：Qi, Xiangyu;Wang, Huadi;Chen, Shuangshuang;Feng, Jing;Chen, Huijie;Qin, Ziyi;Deng, Yanming;Wang, Huadi;Deng, Yanming;Qin, Ziyi;Deng, Yanming;Liu, Shuyun;Chen, Quanming;Blilou, Ikram
关键词：Jasminum sambac Aiton; Oleaceae; Genome evolution; Floral scent; Terpene synthase
