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An efficient measure for the isolation of chenodeoxycholic acid from chicken biles using enzyme-assisted extraction and macroporous resins refining

文献类型： 外文期刊

作者： Yang, Biao 1 ; Lu, Fangyun 1 ; Li, Pengpeng 1 ; Ma, Jingjing 1 ; Yang, Jing 1 ; Zhang, Xinxiao 1 ; Cheng, Mei 1 ; Yu, Wenjing 1 ; Chai, Yao 1 ; Zou, Ye 1 ; Xu, Weimin 1 ; Wang, Daoying 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Agr Prod Cold Chain Logist Technol, Nanjing 210014, Peoples R China

3.Jiangsu Univ, Coll Food & Bioengn, Zhenjiang 212001, Peoples R China

4.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China

关键词： Bile salt hydrolase; Chenodeoxycholic acid; Macroporous resin; Adsorption; Purification

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 1 期

页码：

收录情况： SCI

摘要： Chicken bile is a by-product of chicken processing, rich in chenodeoxycholic acid (CDCA), an active pharmaceutical raw material. In this study, a green measure for the extraction and purification of CDCA from chicken biles by enzymatic hydrolysis and macroporous resins refining was established. For the assisted extraction of CDCA, the active bile salt hydrolase (BSH) from Bifidobacterium was heterologously expressed and applied, its activities on GCDCA and TCDCA were 4.96 +/- 0.32 U/mg and 3.07 +/- 0.031 U/mg and optimal catalytic conditions for the extraction of CDCA were determined as 0.04 g/g of the enzyme dosage, pH 5.0 and 38 degrees C. Through validation of the conditions, the yield of CDCA was up to 5.32 %, which was equivalent to that by saponification method. In order to further refine CDCA from the extract obtained by enzyme-assisted extraction, a more preferable resin, AB-8 was selected for the purification of CDCA, which had a good adsorption capacity of 61.06 +/- 0.57 mg/g for CDCA. Besides, the obtained CDCA extract was purified through AB-8 resin, the purity of CDCA was improved from 51.7 % to 91.4 % and the recovery yield of CDCA was 87.8 %. The advantages of energy conservation, time saving, economy and environmental friendliness make the measure using enzyme-assisted extraction and macroporous resins refining a promising candidate for isolation of CDCA from chicken bile.

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