您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An Improved Procedure for Agrobacterium-Mediated Transformation of 'Carrizo' Citrange

文献类型： 外文期刊

作者： Li, Yanjun 1 ; Tang, Dan 1 ; Liu, Zongrang 3 ; Chen, Jianjun 4 ; Cheng, Baoping 5 ; Kumar, Rahul 1 ; Yer, Huseyin 1 ; Li, Yi 1 ;

作者机构： 1.Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA

2.Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Peoples R China

3.ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV 25430 USA

4.Univ Florida, Midflorida Res & Educ Ctr, Inst Food & Agr Sci, Dept Environm Hort, Apopka, FL 32703 USA

5.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

关键词： treatments of Agrobacterium tumefaciens and explants; acetosyringone; 2-(N-morpholino) ethanesulfonic acid; paclobutrazol; lipoic acid

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2022 年 11 卷 11 期

页码：

收录情况： SCI

摘要： Although several protocols for genetic transformation of citrus have been published, it is highly desirable to further improve its efficiency. Here we report treatments of Agrobacterium cells and citrus explants prior to and during co-cultivation process to enhance transformation efficiency using a commercially used rootstock 'Carrizo' citrange [Citrus sinensis (L.) Osb. x Poncirius trifoliata (L.) Raf.] as a model plant. We found explants from light-grown seedlings exhibited higher transformation efficiency than those from etiolated seedlings. We pre-cultured Agrobacterium cells in a 1/10 MS, 0.5 g/L 2-(N-morpholino) ethanesulfonic acid (MES) and 100 mu M acetosyringone liquid medium for 6 h at 25 degrees C before used to infect citrus explants. We incubated epicotyl segments in an MS liquid medium containing 13.2 mu M 6-BA, 4.5 mu M 2,4-D, 0.5 mu M NAA for 3 h at 25 degrees C prior to Agrobacterium infection. In the co-cultivation medium, we added 30 mu M paclobutrazol and 10 mu M lipoic acid. Each of these treatments significantly increased the efficiencies of transformation up to 30.4% (treating Agrobacterium with acetosyringone), 31.8% (treating explants with cytokinin and auxin), 34.9% (paclobutrazol) and 38.6% (lipoic acid), respectively. When the three treatments were combined, we observed that the transformation efficiency was enhanced from 11.5% to 52.3%. The improvement of genetic transformation efficiency mediated by these three simple treatments may facilitate more efficient applications of transgenic and gene editing technologies for functional characterization of citrus genes and for genetic improvement of citrus cultivars.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台