Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse
文献类型： 外文期刊
作者： Nwamba, Marknoah Chinenye 1 ; Song, Guojie 1 ; Sun, Fubao 1 ; Mukasekuru, Marie Rose 1 ; Ren, Hongyan 2 ; Zhang, Qi 1 ;
作者机构： 1.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China
2.Jiangnan Univ, Jiangsu Key Lab Anaerob Biotechnol, Sch Environm & Civil Engn, Wuxi 214122, Jiangsu, Peoples R China
3.Vland Biotech Ltd Co, Qingdao 266102, Shandong, Peoples R China
4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
5.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China
关键词： Lignocellulosic biomass; Organosolv pretreatment; Enzymatic saccharification; Accessory enzymes and additives; Cellulase cocktail
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.642； 五年影响因子：9.237 ）
ISSN： 0960-8524
年卷期： 2021 年 338 卷
页码：
收录情况： SCI
摘要： The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. The results obtained indicate that the integration of accessory enzymes and additives offers a benignant approach to minimizing the enzyme load and cost of high solids saccharification of lignocellulosic heteropolymers while also boosting enzyme hydrolytic performance.
- 相关文献
作者其他论文 更多>>
-
Enhanced hypoxanthine utilization and antioxidant capacity for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 with glutathione addition
作者：Li, Zhigang;Chen, Baofeng;Ma, Yue;Hua, Chengwei;Chang, Jingling;Sun, Haiyan;Li, Chang;Chang, Jingling;Sun, Haiyan
关键词：CAMP; Glutathione; Oxidative stress; Hypoxanthine; Salvage synthesis
-
Quorum sensing: cell-to-cell communication in Saccharomyces cerevisiae
作者：Li, Linbo;Pan, Yuru;Zhang, Shishuang;Yang, Tianyou;Li, Zhigang;Wang, Baoshi;Zhang, Mingxia;Li, Xu;Sun, Haiyan
关键词：quorum sensing; quorum sensing molecules; quorum sensing system; response mechanism; Saccharomyces cerevisiae
-
Comparative Study of Bolting Adaptability between 60Co-Induced Rape and Its Original Material
作者：Yan, Wei;Tan, Tailong;Chen, Hao;Zhang, Zhenqian;Tan, Tailong;Sun, Haiyan;Hui, Rongkui
关键词：fresh shoot; Co-60; physiological indexes; transcriptomics; metabolomics
-
Preparation and characterization of a laccase-like enzyme from Thermomicrobium roseum
作者：Hao, Mengyao;Huang, Aimin;Li, Bingjie;Xin, Yu;Zhang, Liang;Gu, ZhengHua;Li, Youran;Shi, Guiyang;Hao, Mengyao;Huang, Aimin;Li, Bingjie;Xin, Yu;Zhang, Liang;Gu, ZhengHua;Li, Youran;Shi, Guiyang;Sun, Haiyan;Xin, Yu;Zhang, Liang
关键词：Thermomicrobium roseum Lac -like; Bacillus subtilis; Characterization; Inosine
-
Structure, antioxidant properties, and protective effects on DNA damage of exopolysaccharides from Clostridium butyricum
作者：Cai, Guolin;Lu, Jian;Cai, Guolin;Geng, Yongzhe;Liu, Yifan;Yang, Shaojie;Li, Xiaomin;Lu, Jian;Cai, Guolin;Lu, Jian;Sun, Haiyan;Cai, Guolin
关键词：antioxidant activity; Clostridium butyricum; DNA protection; exopolysaccharide
-
The Bioaccessibility of Yak Bone Collagen Hydrolysates: Focus on Analyzing the Variation Regular of Peptides and Free Amino Acids
作者：Guo, Zitao;Yang, Yuliang;Zhu, Lingyu;Liu, Chunyu;Li, Moying;Gu, Zhenghua;Xin, Yu;Guo, Zhongpeng;Zhang, Liang;Guo, Zitao;Yang, Yuliang;Zhu, Lingyu;Liu, Chunyu;Li, Moying;Gu, Zhenghua;Xin, Yu;Guo, Zhongpeng;Zhang, Liang;Hu, Bo;Sun, Haiyan;Guan, Yanming
关键词：yak bone collagen hydrolysates; bioaccessibility; simulated gastrointestinal digestion and absorption; bioactive peptide; free amino acids
-
Yak bone collagen-derived anti-inflammatory bioactive peptides alleviate lipopolysaccharide-induced inflammatory by inhibiting the NF-KB signaling pathway and nitric oxide production
作者：Yang, Yuliang;Zhu, Lingyu;Guo, Zitao;Liu, Chunyu;Li, Moying;Gu, Zhenghua;Xin, Yu;Zhang, Liang;Yang, Yuliang;Zhu, Lingyu;Guo, Zitao;Liu, Chunyu;Li, Moying;Gu, Zhenghua;Xin, Yu;Zhang, Liang;Hu, Bo;Sun, Haiyan;Guan, Yanming
关键词：Yak bone collagen; Anti-inflammatory peptides; Separation and purification; In silico analysis; Western blot
