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Comparative transcriptome analysis of differentially expressed genes and pathways in Procambarus clarkii (Louisiana crawfish) at different acute temperature stress

文献类型： 外文期刊

作者： Luo, Lei 1 ; Huang, Jian-Hua 1 ; Liu, Dong-Liang 5 ; Jiang, Shi-Gui 1 ; Zhou, Fa-Lin 1 ; Jiang, Song 1 ; Yang, Qi-Bin 4 ; Li, Yun-Dong 1 ; Li, Tao 1 ; Tan, Li-Qi 5 ; Yang, Li-Shi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518108, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploita, Guangzhou 510300, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

5.Shenzhen Anim Dis Prevent & Control Ctr, Shenzhen 518000, Peoples R China

6.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr South China Sea Fis, Sanya 572018, Peoples R China

关键词： Procambarus clarkii; Acute temperature; DEGs; Pathways; Molecular mechanism

期刊名称：GENOMICS （ 影响因子：4.31； 五年影响因子：4.38 ）

ISSN： 0888-7543

年卷期： 2022 年 114 卷 4 期

页码：

收录情况： SCI

摘要： Procambarus clarkii is an important economic species in China, and exhibit heat and cold tolerance in the main culture regions. To understand the mechanisms, we analyzed the hepatopancreas transcriptome of P. clarkii treated at 10 ?, 25 ?, and 30 ?, then 2092 DEGs and 6929 DEGs were found in 30 & DEG;C stress group and 10 ? stress group, respectively. KEGG pathway enrichment results showed that immune pathway is the main stress pathway for 10 ? treatment and metabolic pathway is the main response pathway for 30 ? treatment, which implies low temperature stress induces the damage of the immune system and increases the susceptibility of bacteria while the body response to high temperature stress through metabolic adjustment. In addition, flow cytometry proved that both high and low temperature stress caused different degrees of apoptosis of hemocytes, and dynamic transcription heat map analysis also identified the differential expression of HSPs family genes and apoptosis pathway genes under different heat stresses. This indicates that preventing damaged protein misfolding and accelerating cell apoptosis are necessary mechanisms for P. clarkii to cope with high and low temperature stress. Our research has deepened our understanding of the complex molecular mechanisms of P. clarkii in response to acute temperature stress, and provided a potential strategy for aquatic animals to relieve environmental duress.

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