Rapid Construction and Application of a Vector for Tobacco Ringspot Virus-Induced McPDS Silencing in Bitter Gourd
文献类型： 外文期刊
作者： Zeng, Lingen 1 ; Zhang, Hui 2 ; Guo, Jinju 4 ; Deng, Zhijun 1 ; Liu, Hongbiao 2 ; Du, Hu 2 ; Zhong, Yujuan 2 ; Zhang, Changyuan 4 ; Yao, Chunpeng 2 ;
作者机构： 1.Hubei Minzu Univ, Sch Forestry & Hort, Enshi 445000, Peoples R China
2.Guangdong Acad Agr Sci, Guangdong Key Lab New Technol Res Vegetables, Vegetable Res Inst, Guangzhou 510640, Peoples R China
3.South China Normal Univ, Sch Life Sci, Guangzhou 510631, Peoples R China
4.Guangdong Acad Agr Sci, Inst Facil Agr, Guangzhou 510640, Peoples R China
关键词： bitter gourd; virus-induced gene silencing; TRSV; phytoene dehydrogenase
期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）
ISSN：
年卷期： 2024 年 10 卷 2 期
页码：
收录情况： SCI
摘要： The aim of this study is to facilitate the construction of virus-induced gene silencing vectors and to provide a reference or positive control for gene silencing in bitter gourd. A recombinant TRSV (tobacco ringspot virus) containing two components, pTRSV1 and pTRSV2, was used in this study. The fragment of the McPDS target was cloned into pTRSV2 via combined enzymic ligation during digestion. The TRSV components were agro-infiltrated into tobacco leaves to grow virus particles, which were then extracted and mechanically inoculated into the bitter gourd plants. The effect of TRSV-McPDS-mediated McPDS gene silencing was evaluated by observing the photo-bleaching phenotype, detecting the TRSV virus, and quantifying the downregulation of MCPDS gene expression and chlorophyll contents. The results showed that all bitter gourd plants infected with the empty TRSV or TRSV-McPDS virus grew and developed normally, with no visible signs of viral disease. However, after seven days of inoculation, only the bitter gourd plants that were inoculated with TRSV-McPDS showed obvious photobleaching in the leaves, stems, and buds. The TRSV-specific fragments were tested out in the systemically infected leaves of bitter gourd. The transcription level of the McPDS gene in the leaves dropped by 84.7%. The chlorophyll content also dropped significantly. These data suggest that the rapidly constructed VIGS vector TRSV-McPDS successfully induced McPDS silencing in bitter gourd. Taken together, the results of this study provide a practical method for vector construction in various VIGS applications, as well as a reference and a positive control for TRSV-induced gene silencing in bitter gourd.
- 相关文献
作者其他论文 更多>>
-
Comparing the differences in quality profiles and antioxidant activity in seven pumpkin cultivars ( Cucurbita moschata and Cucurbita maxima) ) at harvest and during postharvest storage
作者：Xu, Yingchao;Wang, Manman;Abbas, Hafiz Muhammad Khalid;Xue, Shudan;Zhu, Jitong;Meng, Qitao;Jin, Qingmin;Zhong, Yujuan;Wang, Manman;Fu, Manqin;Qu, Shuping
关键词：Cucurbita moschata; Cucurbita maxima; Postharvest; Fruit quality; DPPH
-
Transcriptomic analysis reveals the molecular basis of photoperiod-regulated sex differentiation in tropical pumpkins (Cucurbita moschata Duch.)
作者：Xue, Shudan;Huang, Hexun;Xu, Yingchao;Liu, Ling;Meng, Qitao;Zhu, Jitong;Zhou, Meijiang;Du, Hu;Yao, Chunpeng;Jin, Qingmin;Zhong, Yujuan;Meng, Qitao;Nie, Chengrong
关键词：Photoperiod; Cucurbita moschata; Sex differentiation; Photoperiod-mediated flowering processes; Gibberellin signaling pathway; Ethylene biosynthetic and ethylene response pathways
-
Identification of the G2-like transcriptional factor gene family reveals their critical roles in controlling chlorophyll accumulation of melon fruit
作者：Cao, Haishun;Wu, Tingquan;Zhang, Changyuan;Shi, Liangliang;Gong, Mingxi;Anwar, Ali;Song, Shiwei
关键词：G2-like transcription factors; Melon; Chlorophyll; Fruit peel; GLKs; APRR2
-
Genome-wide identification and expression analysis of the cryptochromes reveal the CsCRY1 role under low-light-stress in cucumber
作者：Cao, Haishun;Wang, Rui;Zhao, Junhong;Shi, Liangliang;Wu, Tingquan;Zhang, Changyuan;Huang, Yuan
关键词：cryptochromes; cucumber; low-light-stress; photoreceptors; alternative splicing
-
Potential locus W and candidate gene McPRR2 associated with pericarp pigment accumulation in bitter gourd (Momordica charantia L.) revealed via BSA-seq analysis
作者：Guo, Jinju;Han, Xin;Wu, Tingquan;Wang, Rui;Zhao, Junhong;Wang, Rufang;Tan, Delong;Zhang, Huiyao;Zhang, Changyuan;Yan, Shijuan;Huang, Wenjie;Gao, Jie
关键词：Bitter gourd; BSA-seq; McPRR2; Chlorophyll; Pericarp color
-
The SEPALLATA-like CaSEP5 gene regulates flower sepal, pedicel, and fruit development in pepper (Capsicum annuum L.)
作者：Guo, Jinju;Zhang, Changyuan;Cheng, Qing;Sun, Liang;Shen, Huolin;Shen, Huolin
关键词：SEPALLATA -like genes; Hormone and genetic network; Flower and fruit development; Floral homeotic genes; Capsicum annuum L.
-
Development of comprehensive prediction models for pumpkin fruit sensory quality using physicochemical analysis, near-infrared spectroscopy, and machine learning
作者：Xu, Yingchao;Luo, Jiayu;Xue, Shudan;Jin, Qingmin;Zhu, Jitong;Lu, Sen;Meng, Qitao;Du, Hu;Zhong, Yujuan;Fu, Manqin
关键词：Pumpkin; Quality; Spectroscopy; Machine learning; Prediction model
