High-throughput phenotyping of nutritional quality components in sweet potato roots by near-infrared spectroscopy and chemometrics methods
文献类型： 外文期刊
作者： Tang, Chaochen 1 ; Jiang, Bingzhi 1 ; Ejaz, Irsa 3 ; Ameen, Asif 4 ; Zhang, Rong 1 ; Mo, Xueying 1 ; Wang, Zhangying 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Crops Res Inst, Guangzhou 510640, Peoples R China
2.Key Lab Crop Genet Improvement Guangdong Prov, Guangzhou 510640, Peoples R China
3.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China
4.Pakistan Agr Res Council, Arid Zone Res Ctr, Dera Ismail Khan, Pakistan
关键词： Sweet potato roots; Nutritional quality; Proximate component; Functional component; Near-infrared spectroscopy; Chemometrics
期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.1； 五年影响因子：6.4 ）
ISSN： 2590-1575
年卷期： 2023 年 20 卷
页码：
收录情况： SCI
摘要： The lack of an efficient approach for quality evaluation of sweet potatoes significantly hinders progress in quality breeding. Therefore, this study aimed to establish a near-infrared spectroscopy (NIRS) assay for high-throughput analysis of sweet potato root quality, including total starch, amylose, amylopectin, the ratio of amylopectin to amylose, soluble sugar, crude protein, total flavonoid content, and total phenolic content. A total of 125 representative samples were utilized and a dual-optimized strategy (optimization of sample subset partitioning and variable selection) was applied to NIRS modeling. Eight optimal equations were developed with an excellent coefficient of determination for the calibration (R2C) at 0.95-0.99, cross-validation (R2CV) at 0.93-0.98, external validation (R2V) at 0.89-0.96, and the ratio of prediction to deviation (RPD) at 6.33-11.35. Overall, these NIRS models provide a feasible approach for high-throughput analysis of root quality and permit large-scale screening of elite germplasm in future sweet potato breeding.
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