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Global survey of alternative splicing and gene modules associated with fertility regulation in a thermosensitive genic male sterile wheat

文献类型： 外文期刊

作者： Bai, Jianfang 1 ; Wang, Yukun 1 ; Liu, Zihan 1 ; Guo, Haoyu 1 ; Zhang, Fengting 1 ; Guo, Liping 1 ; Yuan, Shaohua 1 ; Duan, Wenjing 1 ; Li, Yanmei 1 ; Tan, Zhaoguo 1 ; Zhao, Changping 1 ; Zhang, Liping 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Engn Res Ctr Hybrid Wheat, Beijing 100097, Peoples R China

2.Municipal Key Lab Mol Genet Hybrid Wheat, Beijing 10097, Peoples R China

3.Nara Inst Sci & Technol, Grad Sch Sci & Technol, Div Biol Sci, Nara 6300192, Japan

4.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China

5.Shaoguan Univ, Henry Fok Sch Biol & Agr, Shaoguan 512000, Peoples R China

关键词： Alternative splicing; low temperature; male sterility; PacBio sequencing; RNA-Seq; wheat

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：7.298； 五年影响因子：8.291 ）

ISSN： 0022-0957

年卷期： 2022 年 73 卷 7 期

页码：

收录情况： SCI

摘要： Thermosensitive genic male sterile (TGMS) wheat lines are the core of two-line hybrid systems. Understanding the mechanism that regulates male sterility in TGMS wheat lines is helpful for promoting wheat breeding. Several studies have obtained information regarding the mechanisms associated with male sterility at the transcriptional level, but it is not clear how the post-transcriptional process of alternative splicing might contribute to controlling male sterility. In this study, we performed genome-wide analyses of alternative splicing during the meiosis stage in TGMS line BS366 using PacBio and RNA-Seq hybrid sequencing. Cytological observations indicated that cytoskeleton assembly in pollen cells, calcium deposition in pollen and tapetal cells, and vesicle transport in tapetal cells were deficient in BS366. According to our cytological findings, 49 differentially spliced genes were isolated. Moreover, 25 long non-coding RNA targets and three bHLH transcription factors were identified. Weighted gene co-expression network analysis detected four candidate differentially spliced genes that had strong co-relation with the seed setting percentage, which is the direct representation of male sterility in BS366. In this study, we obtained comprehensive data regarding the alternative splicing-mediated regulation of male sterility in TGMS wheat. The candidates identified may provide the molecular basis for an improved understanding of male sterility. Comprehensive analysis of alternative splicing events and differentially spliced genes in a thermosensitive genic male sterile wheat line identifies fertility-related gene modules, lncRNAs, and transcription factors.

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