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Morphological and transcriptomic analysis of attenuated and virulent strains of Phytophthora infestans

文献类型： 外文期刊

作者： Liu, Jiafang 1 ; Li, Lei 2 ; Wang, Yong 3 ; Yun, Xingfu 1 ;

作者机构： 1.Inner Mongolia Agr Univ, Coll Hort & Plant Protect, 306 Zhaowuda Rd, Hohhot 010018, Peoples R China

2.Inner Mongolia Univ, Manzhouli Russian Vocat & Tech Coll, Manzhouli Coll, Hulun Buir 021400, Peoples R China

3.Inner Mongolia Acad Agr & Anim Husb Sci, Vegetable Res Inst, Hohhot 010010, Peoples R China

关键词： Phytophthora infestans; Attenuated strain; Growth and pathogenicity; RNA-seq; Bioinformatics analysis; DEGs

期刊名称：INTERNATIONAL MICROBIOLOGY （ 影响因子：2.479； 五年影响因子：2.266 ）

ISSN： 1139-6709

年卷期：

页码：

收录情况： SCI

摘要： Phytophthora infestans is a hemibiotroph Oomycete that primarily infects tomato. In this study, the growth status and pathogenicity of attenuated and virulent strains of Phytophthora infestans were determined. Furthermore, RNA-seq technology was used to explore the differences in gene transcription levels between attenuated and virulent strains. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on the differentially expressed genes (DEGs) obtained by sequencing, and the significant DEGs related to the growth and pathogenicity of the strains were screened from the significantly enriched pathways. The results revealed that compared with the virulent strain, the growth of the attenuated strain was inhibited, the structure of hyphae was destroyed, and the disease index was decreased. The differences in the growth status and disease index of the attenuated strain were related to changes in several metabolic pathways, and the DEGs in the metabolic pathways indicated alterations in the attenuated strain growth and pathogenicity. There were 2,651 DEGs in the attenuated strain, of which 1,086 were upregulated and 1,565 were downregulated. The inhibited growth of the attenuated strain was associated with accumulation of excessive glucose, decomposition of serine/glycine, and reduction of tryptophan synthesis. The reduced pathogenicity of the strain was associated with degradation of the cell wall and reduced formation of melanin and alpha-keto butyric acid. These results could offer insights into the mechanisms of attenuation of Phytophthora infestans.

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