Screening white spot syndrome virus (WSSV)-resistant molecular markers from Fenneropenaeus chinensis
文献类型： 外文期刊
作者： Wu Yingying 1 ; Meng Xianhong 2 ; Kong Jie 2 ; Luan Sheng 2 ; Luo Kun 2 ; Wang Qingyin 2 ; Zheng Yongyun 1 ;
作者机构： 1.Marine Biol Inst Shandong Prov, Qingdao 266002, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Re
关键词： Fenneropenaeu chinensis; WSSV; SNP; molecular marker
期刊名称：JOURNAL OF OCEAN UNIVERSITY OF CHINA （ 影响因子：0.913； 五年影响因子：1.012 ）
ISSN： 1672-5182
年卷期： 2017 年 16 卷 1 期
页码：
收录情况： SCI
摘要： White spot syndrome virus (WSSV)-resistant molecular markers were screened from the selectively bred new variety 'Huanghai No. 2' of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting (HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and -resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms (SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89AT, genotype A/A of C2635-527CA and genotype C/T of contig C12355-592CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145AG and genotype C/C of contig C12355-592CT, were negatively correlated. Moreover, analysis with BlastX program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis.
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