您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification, characterization of selenoprotein W and its mRNA expression patterns in response to somatostatin 14, cysteamine hydrochloride, 17 beta-estradiol and a binary mixture of 17 beta-estradiol and cysteamine hydrochloride in topmouth culter (Erythroculter ilishaeformis)

文献类型： 外文期刊

作者： Dong, Haiyan 1 ; Chen, Wenbo; Sun, Chao 1 ; Sun, Jianwei 1 ; Wang, Yanlin 1 ; Xie, Chao 1 ; Fu, Qianwen 1 ; Zhu, Junji 1 ;

作者机构： 1.Huzhou Univ, Dept Basic Med Sci, 759 Erhuan East Rd, Huzhou 313000, Zhejiang, Peoples R China

2.Chinese Acad Fishery Sci, National Local Joint Engn Lab Aquat Anim Genet Br, Zhejiang Prov Key Lab Aquat Resources Conservat &, Key Lab Aquat Anim Genet Breeding & Nutr, 759 Erhuan East Rd, Huzhou 313000, Zhejiang, Peoples R China

3.Chinese Acad Fishery Sci, National Local Joint Engn Lab Aquat Anim Genet Br, Zhejiang Prov Key Lab Aquat Resources Conservat &, Key Lab Aquat Anim Genet Breeding & Nutr, 759 Erhuan East Rd, Huzhou 313000, Zhejiang, Peoples

关键词： Selenoprotein W; Somatostatin 14; Cysteamine hydrochloride; 17 beta-Estradiol; Injection

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2017 年 43 卷 1 期

页码：

收录情况： SCI

摘要： In this study, a selenoprotein W cDNA was cloned from topmouth culter (Erythroculter ilishaeformis), and it was designated as EISelW. The EISelW open reading frame was composed of 261 base pairs (bp), encoding 86-amino-acid protein. The 5' untranslated region (UTR) consisted of 104 bp, and the 3'-UTR was composed of 365 bp. A selenocysteine insertion sequence (SECIS) element was found in the 3'-UTR of EISelW mRNA. The SECIS element was classified as form II because of a small additional apical loop presented in SECIS element of EISelW mRNA. Bioinformatic approaches showed that the secondary structure of EISelW was a beta 1-alpha 1-beta 2-beta 3-beta 4-alpha 2 pattern from amino-terminal to carboxy-terminal. Real-time PCR analysis of EISelW mRNAs expression in 17 tissues showed that the EISelW mRNA was predominantly expressed in liver, ovary, pituitary, various regions of the brain, spinal cord and head kidney. Study of intraperitoneal injection showed that the levels of EISelW mRNA in brain, liver, ovary and spleen were regulated by somatostatin 14 (SS14), 17 beta-estradiol (E2), cysteamine hydrochloride (CSH) and a binary mixture of E2 and CSH, dependent on the dosage. These results suggest that E2, SS14 and CSH status may affect tissues of selenium metabolism by regulating the expression of SelW mRNA, as SelW plays a central role in selenium metabolism.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台