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Differentially expressed immune-related genes in hemocytes of the pearl oyster Pinctada fucata against allograft identified by transcriptome analysis

文献类型： 外文期刊

作者： Wei, Jinfen 1 ; Liu, Baosuo 2 ; Fan, Sigang 2 ; Li, Haimei 2 ; Chen, Mingqiang 2 ; Zhang, Bo 2 ; Su, Jiaqi 2 ; Meng, Ziha 1 ;

作者机构： 1.Qinzhou Univ, Qinzhou 535011, Peoples R China

2.South China Sea Resource Exploitat & Protect Coll, Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China

4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Pinctada fucata; Allotransplantation; Immune response; Pearl culture; Transcriptome

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2017 年 62 卷

页码：

收录情况： SCI

摘要： The pearl oyster Pinctada fucata is commonly cultured for marine pearls in China. To culture pearls, a mantle piece from a donor pearl oyster is grafted with a nucleus into a receptor. This transplanted mantle piece may be rejected by the immune system of the recipient oyster, thus reducing the success of transplantation. However, there have been limited studies about the oyster's immune defense against allograft. In this study, hemocyte transcriptome analysis was performed to detect the immune responses to allograft in P. fucata at 0 h and 48 h after a transplant. The sequencing reaction produced 92.5 million reads that were mapped against the reference genome sequences of P. fucata. The Gene Ontology (GO) annotation and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to identify all immune related differentially expressed genes (DEGs). Compared with patterns at 0 h, a total of 798 DEGs were identified, including 410 up-regulated and 388 down-regulated genes at 48 h. The expression levels of interleukin receptor and toll-like receptor in hemocytes were increased significantly 48 h post transplant, indicating that the oyster immune response was induced. Finally, altered levels of 18 randomly selected immune-related DEGs were confirmed by quantitative real-time PCR (qRT-PCR). Our results provide the basis for further analysis of the immune rejection of allotransplantation. (C) 2017 Elsevier Ltd. All rights reserved.

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