Rapid Visual Identification of Bovine Meat by Loop Mediated Isothermal Amplification Combined with Immunochromatographic Strip
文献类型： 外文期刊
作者： Li, Yong-Jin 1 ; Fang, Jin-Yu 2 ;
作者机构： 1.Huzhou Univ, Chinese Acad Fishery Sci, Key Lab Aquat Anim Genet Breeding & Nutr, Zhejiang Prov Key Lab Aquat Resources Conservat &, Huzhou 313000, Peoples R China
2.Xinxiang Med Univ, Coll Life Technol, Xinxiang 453003, Peoples R China
关键词： LAMP; Immunochromatographic strip; Species identification; PCR; Colorimetric analysis
期刊名称：BIOCHIP JOURNAL （ 影响因子：3.494； 五年影响因子：2.325 ）
ISSN： 1976-0280
年卷期： 2017 年 11 卷 1 期
页码：
收录情况： SCI
摘要： Considering the factor of culture, religion, health and commerce, the development of a rapid, convenient and sensitive method is important for the detection of the presence of meat species in raw or processed foods. In this study, we employed loop mediated isothermal amplification (LAMP) for species-specific target gene amplification, and the amplicons were analyzed on the immunochromatographic strip (ICS) for the rapid visual identification of meat species. Bovine meat was selected as an example to confirm the performance of such assay. The LAMP based ICS method show good specificity, repeatability and has a limit of detection of 0.1% for beef in meat mixture. The whole detection process could be completed within 50 min. Our method: is more convenient,and rapid compared to classicaqAMP. Moreover, it could be, easily adapted to identify types of meats, and it may be useful for food administration laboratories to carry out meat species identification idiaw. and processed foods.
- 相关文献
