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Intestine oxidative stress and immune response to sulfide stress in Pacific white shrimp Litopenaeus vannamei

文献类型： 外文期刊

作者： Duan, Yafei 1 ; Dong, Hongbiao 1 ; Wang, Yun 1 ; Li, Hua 1 ; Liu, Qingsong 1 ; Zhang, Yue 1 ; Zhang, Jiasong;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

关键词： Crustaceans; Environmental stress; Acute toxicity; Oxidative stress; Immune enzymes; Gene expression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2017 年 63 卷

页码：

收录情况： SCI

摘要： The effects of sulfide stress on oxidative stress and immune response in intestine of Pacific white shrimp Litopenaeus vannamei were evaluated in the present study. Oxidative stress parameters, immune enzymes activity and immune gene mRNA expression level were detected in intestine of L. vannamei after the exposure of 5.0 mg/L sulfide stress 72 h. The duration of sulfide stress influenced the shrimp survival, and the cumulative mortality rate was 30.0% and 333% at 48 h and 72 h respectively. HE staining showed that sulfide stress caused the intestine tissue damage symptoms. Compared with the control group, after exposed to sulfide stress, the content of lipid peroxidation (LPO), malondialdehyde (MDA) and ROS production (center dot O-2(-) generation capacity) increased. Total antioxidant capacity (T-AOC) activity increased at 6 h and decreased at 48 h. Superoxide dismutase (SOD) activity increased in the entire experiment. Inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content increased to the highest at 6 h and 12 h respectively, and both decreased at 48 h. The relative mRNA expression level of heat shock protein 70 (HSP70) gene decreased at 6 h and increased to the highest at 48 h. The relative mRNA expression level of hypoxia inducible factor la (HIF-1a) gene increased at 12 h and decreased to a lower level at 72 h. The relative mRNA expression level of Toll and immune deficiency (Imd) gene increased to the highest at 12 h and 24 h respectively, and both decreased at 48 h. These results revealed that sulfide stress could induce oxidative stress and immune response via confusion of immune enzymes activity and gene expression level in intestine of L. vannamei. (C) 2017 Elsevier Ltd. All rights reserved.

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