Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)
文献类型: 外文期刊
作者: Shao, Jian 1 ; Luo, Wei 4 ; Wei, Qiwei 1 ; Wang, Feng 5 ; Guo, Wei 2 ; Ye, Huan 2 ; Chu, Zhipeng 2 ; Wu, Jinming 2 ; Zhang, 1 ;
作者机构: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
3.Guizhou Univ, Coll Anim Sci, Guiyang 550025, Peoples R China
4.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
5.Chinese Acad Fishery Sci, Yellow River Fisheries Res Inst, Xian 710086, Peoples R China
关键词: Brachymystax lenok tsinlingensis; Microsatellites; Parentage assignment; Inbreeding depression
期刊名称:AQUATIC BIOLOGY ( 影响因子:1.8; 五年影响因子:1.945 )
ISSN: 1864-7790
年卷期: 2017 年 26 卷
页码:
收录情况: SCI
摘要: We evaluated the use of microsatellite markers to identify parentage in Brachy mystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellitebased parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.
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