Overexpression of Loquat Dehydrin Gene EjDHN1 Promotes Cold Tolerance in Transgenic Tobacco
文献类型： 外文期刊
作者： Xu, H. X. 1 ; Li, X. Y. 1 ; Xu, C. J. 2 ; Chen, J. W. 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Univ, Lab Fruit Qual Biol, State Agr Minist Lab Hort Plant Growth Dev & Qual, Zijingang Campus, Hangzhou, Zhejiang, Peoples R China
关键词： Eriobotrya japonica; EjDHN1; cold stress; oxidative stress; transgenic; tobacco
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）
ISSN： 1021-4437
年卷期： 2018 年 65 卷 1 期
页码：
收录情况： SCI
摘要： Dehydrins (DHNs) play vital roles in response to dehydration stress in plants. To examine the contribution of EjDHN to low-temperature stress in loquat (Eriobotrya japonica Lindl.), EjDHN1 was overexpressed in tobacco (Nicotiana tabacum L.). The plant growth of transgenic lines was significantly better than wild type (WT) after 4 d of recovery from cold stress. Cold stress led to membrane lipid peroxidation and reduced photosystem II (PSII) activity in leaves, and these were less severe in transgenic lines. To examine oxidative stress tolerance, the plants were treated with different concentrations of methyl viologen (MV), which inhibited plant growth both in WT and transgenic lines. After exposure to 2.0 mu M MV for 10 d, the WT plants had a dramatically lower survival rate. MV treatment in leaf disks confirmed that transgenic lines accumulated less reactive oxygen species (ROS) and suffered less lipid peroxidation. The results suggested that the tolerance of the transgenic plants to cold was increased, and EjDHN1 could protect cells against oxidative damage caused by ROS production under cold stress. It also provided evidences that the enhanced cold tolerance resulted from EjDHN1 overexpression could be partly due to their protective effect on membranes by alleviating oxidative stresses.
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