Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy
文献类型： 外文期刊
作者： Li, X. Y. 1 ; Xu, H. X. 1 ; Chen, J. W. 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China
关键词： Loquat;EST-SSR;Marker;Polymorphic;Manual cultivar identification diagram
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2014 年 13 卷 2 期
页码：
收录情况： SCI
摘要： Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products. After analysis of 24 red-fleshed loquat accessions, we found that only 7 pairs of primers could clearly separate all of them. A cultivar identification diagram of the 24 cultivars was constructed using polymorphic bands from the DNA fingerprints and EST-SSR primers. Any two of the 24 cultivars could be rapidly separated from each other, according to the polymorphic bands from the cultivars; the corresponding primers were marked in the correct position on the cultivar identification diagram. This red-flesh loquat cultivar identification diagram can separate the 24 red-flesh loquat cultivars, which is of benefit for loquat cultivar identification for germplasm management and breeding programs.
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