Differential Expression of Six Rnase2 and Three Rnase3 Paralogs Identified in Blunt Snout Bream in Response to Aeromonas hydrophila Infection
文献类型： 外文期刊
作者： Geng, Ruijing 1 ; Liu, Han 1 ; Wang, Weimin 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ,Key Lab Freshwater Anim Breeding, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China
3.Collaborat Innovat Ctr Efficient & Hlth Prod Fish, Changde 415000, Peoples R China
关键词： blunt snout bream; Rnase2; Rnase3; expression patterns; biological function
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN： 2073-4425
年卷期： 2018 年 9 卷 2 期
页码：
收录情况： SCI
摘要： Ribonucleases (Rnases)2 and Rnase3 belong to the ribonuclease A (RnaseA) superfamily. Apart from their role in molecular evolutionary and functional biological studies, these genes have also been studied in the context of defense against pathogen infection in mammals. However, expression patterns, structures and response to bacterial infection of the two genes in blunt snout bream (Megalobrama amblycephala) remain unknown. In this study, we identified multiple copies of Rnase2 (six) and Rnase3 (three) in the M. amblycephala genome. The nine genes all possess characteristics typical of the RnaseA superfamily. No expression was detected in the early developmental stages, while a weak expression was observed at 120 and 140 h post-fertilization (hpf) for Rnase2b, Rnase2c, Rnase2e and Rnase3a, suggesting that only three copies of Rnase2 and one of Rnase3 are expressed. Interestingly, only Rnase2e was up-regulated in the kidney of M. amblycephala after Aeromonas hydrophila infection, while Rnase3a was significantly up-regulated in liver, gut and blood after the infection. We conclude that the paralogs of Rnase3 are more susceptible to A. hydrophila infection than Rnase2. These results indicate that different Rnase2 and Rnase3 paralogs suggest a role in the innate immune response of M. amblycephala to bacterial infection.
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