Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
文献类型： 外文期刊
作者： Qiong, Yang 1 ; Xu, Xing Dong 1 ; Rong, Li Qing 1 ; Yang, Xiao 1 ; Qiang, Ye Ming 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Sericulture & Agrifood Res Inst, Guangzhou 510610, Guangdong, Peoples R China
关键词： Bombyx mori nucleopolyhedrovirus; Group inoculation method; Individual inoculation method; Resistance detection; Silkworm
期刊名称：REVISTA BRASILEIRA DE ENTOMOLOGIA （ 影响因子：1.133； 五年影响因子：1.3 ）
ISSN： 0085-5626
年卷期： 2018 年 62 卷 1 期
页码：
收录情况： SCI
摘要： Bombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an effective way to improve resistance to BmNPV and reduce economic losses. A precise resistance-detection method will help to accelerate the breeding process. For this purpose, here we described the individual inoculation method (IIM). Details of the IIM include pathogen BmNPV preparation, mulberry leaf size, pathogen volume, rearing conditions, course of infection, and breeding conditions. Finally, a resistance comparison experiment was performed using the IIM and the traditional group inoculation method (GIM). The incidence of BmNPV infection and the within-group variance results showed that the IIM was more precise and reliable than the GIM. (C) 2017 Sociedade Brasileira de Entomologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license.
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