浙江省农业科学院机构知识库

De Novo Transcriptome Assembly and Characterization of the Synthesis Genes of Bioactive Constituents in Abelmoschus esculentus (L.) Moench

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文献类型：外文期刊

作者： Zhang, Chenghao ¹ ; Dong, Wenqi ¹ ; Gen, Wei ¹ ; Xu, Baoyu ¹ ; Shen, Chenjia ³ ; Yu, Chenliang ¹ ;

作者机构： 1.Zhejiang Acad Agr Sci, Vegetable Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Key Lab Creat Agr, Minist Agr, Hangzhou 310021, Zhejiang, Peoples R China

3.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China

关键词： flavonoids; glycosyltransferase; polysaccharide; okra; transcriptome

期刊名称：GENES （影响因子：4.096；五年影响因子：4.339 ）

ISSN： 2073-4425

年卷期： 2018 年 9 卷 3 期

页码：

收录情况： SCI

摘要： Abelmoschus esculentus (okra or lady's fingers) is a vegetable with high nutritional value, as well as having certain medicinal effects. It is widely used as food, in the food industry, and in herbal medicinal products, but also as an ornamental, in animal feed, and in other commercial sectors. Okra is rich in bioactive compounds, such as flavonoids, polysaccharides, polyphenols, caffeine, and pectin. In the present study, the concentrations of total flavonoids and polysaccharides in five organs of okra were determined and compared. Transcriptome sequencing was used to explore the biosynthesis pathways associated with the active constituents in okra. Transcriptome sequencing of five organs (roots, stem, leaves, flowers, and fruits) of okra enabled us to obtain 293,971 unigenes, of which 232,490 were annotated. Unigenes related to the enzymes involved in the flavonoid biosynthetic pathway or in fructose and mannose metabolism were identified, based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. All of the transcriptional datasets were uploaded to Sequence Read Archive (SRA). In summary, our comprehensive analysis provides important information at the molecular level about the flavonoid and polysaccharide biosynthesis pathways in okra.

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