Genome-wide association analysis reveals the optimal genomic regions for pod size in bean
文献类型： 外文期刊
作者： Li, Mao 1 ; Wu, Xinyi 2 ; Wang, Baogen 2 ; Wu, Xiaohua 2 ; Wang, Ying 2 ; Wang, Jian 2 ; Dong, Junyang 1 ; Wu, Jian 1 ; Lu, Zhongfu 2 ; Sun, Yuyan 2 ; Dong, Wenqi 2 ; Yang, Jing 1 ; Li, Guojing 2 ;
作者机构： 1.Zhejiang A&F Univ, Coll Hort Sci, Collaborat Innovat Ctr Efficient & Green Prod Agr, Key Lab Qual & Safety Control Subtrop Fruit & Vege, Hangzhou, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou, Peoples R China
3.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China
4.Zhejiang Acad Agr Sci, Coconstruct Minist & Prov, Minist Agr & Rural Affairs, Key Lab Vegetable Legumes Germplasm Enhancement &, Hangzhou, Peoples R China
关键词： pod size; GWAS; KASP marker; quality; snap bean
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： The snap bean is the most commonly grown vegetable legume worldwide, and its pod size is both an important yield and appearance quality trait. However, the improvement of pod size in snap beans grown in China has been largely hindered by a lack of information on the specific genes that determine pod size. In this study, we identified 88 snap bean accessions and evaluated their pod size traits. Through a genome-wide association study (GWAS), 57 single nucleotide polymorphisms (SNPs) significantly associated with pod size were detected. Candidate gene analysis showed that cytochrome P450 family genes, WRKY, and MYB transcription factors were the predominant candidate genes for pod development, and eight of these 26 candidate genes showed relatively higher expression patterns in flowers and young pods. A significant pod length (PL) SNP and a single pod weight (SPW) SNP were successfully converted into kompetitive allele-specific polymerase chain reaction (KASP) markers and validated in the panel. These results enhance our understanding of the genetic basis of pod size, and also provide genetic resources for the molecular breeding of pod size in snap beans.
- 相关文献
作者其他论文 更多>>
-
A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection
作者：Yang, Lei;Chen, Guanwei;Wei, Wei;Peng, Cheng;Ding, Lin;Chen, Xiaoyun;Xu, Xiaoli;Wang, Xiaofu;Xu, Junfeng;Chen, Guanwei;Wu, Jian
关键词：
-
A new simplified sequence-dependent loop-mediated isothermal amplification (LAMP) detection method
作者：Chen, Yanju;Zhu, Yuanyuan;Du, Jungang;Wu, Jian;Chen, Yanju;Zhu, Yuanyuan;Du, Jungang;Wu, Jian;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng;Zhou, Qingli;Chen, Huan
关键词：Isothermal amplification; LAMP; Sequence-dependent probe; Multiplexed detection; Diagnostics
-
Differential selection of yield and quality traits has shaped genomic signatures of cowpea domestication and improvement
作者：Wu, Xinyi;Li, Mao;Dong, Junyang;Wang, Baogen;Wu, Jian;Wu, Xiaohua;Wang, Ying;Wang, Jian;Lu, Zhongfu;Sun, Yuyan;Dong, Wenqi;Li, Guojing;Hu, Zhongyuan;Liao, Nanqiao;Zhang, Mingfang;Zhang, Yan;Yang, Yi;Li, Mao;Dong, Junyang;Wang, Baogen;Wu, Jian;Wu, Xiaohua;Wang, Ying;Wang, Jian;Lu, Zhongfu;Sun, Yuyan;Dong, Wenqi;Li, Guojing;Zhang, Mingfang;Zhang, Mingfang
关键词：
-
Stomatal evolution and plant adaptation to future climate
作者：Chen, Guang;Wang, Jian;Li, Sujuan;Xu, Shengchun;Qin, Yuan;Zeng, Fanrong;Deng, Fenglin;Chater, Caspar;Chater, Caspar;Xu, Shengchun;Chen, Zhong-Hua;Chen, Zhong-Hua
关键词：abiotic stresses; climate change; membrane transporters; molecular evolution; photosynthesis; stomatal guard cell; sustainable plant production
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者：Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词：bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress
-
A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane
作者：Chen, Yanju;Zhu, Yuanyuan;Wu, Jian;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng;Chen, Huan
关键词：nucleic acid quantification; digital detection; paper membrane; probe-based LAMP
-
Effect of two different Flammulina velutipes polysaccharides on the physicochemical and digestive properties of rice starch and their interaction mechanisms
作者：Bu, Tingting;Ren, Yuting;Yu, Yue;Lu, Jun;Wang, Jian;Cai, Ming;Yang, Kai;Sun, Peilong;Bu, Tingting;Ren, Yuting;Yu, Yue;Wang, Jian;Cai, Ming;Yang, Kai;Sun, Peilong;Wu, Weicheng;Zhang, Zhiguo;Hu, Weiwei;Lu, Jun
关键词：Flammulina velutipes; Polysaccharides; Rice starch; Pasting; Gelatinization; Starch digestion
