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A QuEChERS-Based Liquid Chromatography-Tandem Mass Spectrometry Method for the Simultaneous Determination of Nine Zearalenone-Like Mycotoxins in Pigs

文献类型： 外文期刊

作者： Yan, Zheng 1 ; Wang, Lan 1 ; Wang, Jun 2 ; Tan, Yanglan 1 ; Yu, Dianzhen 1 ; Chang, Xiaojiao 2 ; Fan, Yingying 3 ; Zhao, 1 ;

作者机构： 1.Chinese Acad Sci, Univ Chinese Acad Sci, Key Lab Food Safety Res, SIBS UGENT SJTU Joint Lab Mycotoxin Res,Shanghai, Shanghai 200000, Peoples R China

2.Acad State Adm Grain PRC, 11 Baiwanzhuang Ave, Beijing 100037, Peoples R China

3.Xinjiang Acad Agr Sci, Key Lab Agroprod Qual & Safety Xinjiang, Lab Qual & Safety Risk Assessment Agroprod Urumqi, Minist Agr,Inst Qual Stand & Testing Technol Agro, Urumqi 830091, Peoples R China

4.Univ Ghent, Fac Pharmaceut Sci, Dept Bioanal, Lab Food Anal, B-9000 Ghent, Belgium

关键词： zearalenone; LC-MS/MS; QuEChERS; pigs

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2018 年 10 卷 3 期

页码：

收录情况： SCI

摘要： The determination of zearalenone (ZEN) and its derivatives as biomarkers in animal tissues or organs plays an important role in mycotoxin monitoring and can promote effective exposure assessment. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of nine ZEN-like mycotoxins, including three glucuronides in different pig tissues (heart, liver, spleen and muscle) was developed and validated in this study. Tissue samples were extracted using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction and clean-up procedure, and analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode. Dynamic linear ranges for each target analyte were determined with R-2 between 0.916 and 0.999. The LODs of the six ZENs were achieved in the range of 0.5-1 ng/g and the LOQs varied from 1 ng/g to 2 ng/g. The satisfying intra-day and inter-day reproducibility (both RSDr and RSDR < 20%) indicated a good stability of this method. The recoveries of the nine target analytes were in the range of 70-110%. The validation results showed that this LC-MS/MS method coupled with QuEChERS sample pretreatment is effective and suitable for the simultaneous quantitation of ZEN metabolites in pigs. It has been applied to analysis of the pig tissues in this research and can be also adapted for samples in the mycotoxin research field.

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