Molecular and functional characterization of ShNAC1, an NAC transcription factor from Solanum habrochaites
文献类型： 外文期刊
作者： Liu, Hui 1 ; Zhou, Yuhong 2 ; Li, Hanxia 2 ; Wang, Taotao 2 ; Zhang, Junhong 2 ; Ouyang, Bo 2 ; Ye, Zhibiao 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Minist Agr, Danzhou 571737, Peoples R China
2.Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Hubei, Peoples R China
关键词： Tomato; Solanum habrochaites; Abiotic stress; NAC transcription factor; Senescence; Ethylene
期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）
ISSN： 0168-9452
年卷期： 2018 年 271 卷
页码：
收录情况： SCI
摘要： NAC transcription factors (TFs) are important regulators of plant adaptation to abiotic stress. In this study, we functionally characterized an NAC TF, ShNAC1, from Solanum habrochaites. ShNAC1 was up-regulated by drought, cold, and salt stresses, and it displayed lower expression at the late stage of stress treatments than its orthologous gene in S. lycopersicum. Overexpression of ShNAC1 in tomato resulted in reduced cold, drought, and salt tolerance. Additionally, ShNAC1 displayed the highest expression in senescent leaf, and overexpressing ShNAC1 accelerated salt- and dark-induced leaf senescence. ShNAC1 was located in the nucleus without transactivation activity. RNA-seq analysis revealed that 81% (190 out of 234) differentially-expressed genes (DEGs) showed down-regulation in the transgenic line L2 compared with wild-type, suggesting that ShNAC1 may function as a transcriptional repressor. Among these down-regulated DEGs, many were involved in stress responses, such as SIHKT1;1, SIMAPKKK59, SIJA2, SITIL, SIALDH2B1, etc. Noticeably, one ACS gene and three ACO genes involved in ethylene biosynthesis were up-regulated, while seven ERF genes in the ethylene signal transduction pathway were down-regulated in the transgenic lines, respectively. Our results suggested that ShNAC1 negatively regulates tolerance to abiotic stress in tomato probably by modulating the ethylene biosynthesis and signal transduction pathways.
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