RNA interference of GhPEPC2 enhanced seed oil accumulation and salt tolerance in Upland cotton
文献类型： 外文期刊
作者：
Zhao, Yanpeng
1
;
Huang, Yi
2
;
Wang, Yumei
3
;
Cui, Yupeng
1
;
Liu, Zhengjie
1
;
Hua, Jinping
1
;
作者机构： 1.China Agr Univ, Coll Agron & Biotechnol, Lab Cotton Genet Genom & Breeding,Minist Educ, Beijing Key Lab Crop Genet Improvement,Key Lab Cr, Beijing 100193, Peoples R China
2.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Hubei, Peoples R China
3.Hubei Acad Agr Sci, Res Inst Cash Crops, Wuhan 430064, Hubei, Peoples R China
关键词： GhPEPC2; RNA interference; Oil content; Salt stress; RNA-Seq; Upland cotton
期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）
ISSN： 0168-9452
年卷期： 2018 年 271 卷
页码：
收录情况： SCI
摘要： Phosphoenolpyruvate carboxylase (PEPCase) mainly produces oxaloacetic acid for tricarboxylic acid (TCA) cycle. Here we reported that GhPEPC2 silencing with PEPC2-RNAi vector could regulate oil and protein accumulation in cottonseeds. In GhPEPC2 transgenic plants, PEPCase activities in immature embryos were significantly reduced, and the oil content in seed kernel was increased 7.3 percentages, whereas total proteins decreased 5.65 percentages. Compared to wild type, agronomical traits of transgenic plant were obviously unaffected. Furthermore, gene expression profile of GhPEPC2 transgenic seeds were investigated using RNA-seq, most lipid synthesis related genes were up-regulated, but amino acid metabolic related genes were down regulated. In addition, the GhPEPC2 transgenic cotton seedlings were stressed using sodium salts at seedling stage, and the salt tolerance was significantly enhanced. Our observations of GhPEPC2 in cotton would shade light on understanding the regulation of oil content, protein accumulation and salt tolerance enhancement in other plants.
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