文献类型： 外文期刊

作者： Tang Xianghai ¹ ; Xu Kuipeng ¹ ; Han Xiaojuan ¹ ; Mo Zhaolan ² ; Mao Yunxiang ¹ ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China

关键词： Cobetia marina JCM 21022(T); Halomonadaceae; complete genome sequence; comparative genomics; phylogenomics; surface colonization; single molecule real-time sequencing technology (SMRT)

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 2020影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2018 年 36 卷 2 期

页码：

收录情况： SCI

摘要： Cobetia marina is a model proteobacteria in researches on marine biofouling. Its taxonomic nomenclature has been revised many times over the past few decades. To better understand the role of the surface-associated lifestyle of C. marina and the phylogeny of the family Halomonadaceae, we sequenced the entire genome of C. marina JCM 21022(T) using single molecule real-time sequencing technology (SMRT) and performed comparative genomics and phylogenomics analyses. The circular chromosome was 4 176 300 bp with an average GC content of 62.44% and contained 3 611 predicted coding sequences, 72 tRNA genes, and 21 rRNA genes. The C. marina JCM 21022(T) genome contained a set of crucial genes involved in surface colonization processes. The comparative genome analysis indicated the significant differences between C. marina JCM 21022(T) and Cobetia amphilecti KMM 296 (formerly named C. marina KMM 296) resulted from sequence insertions or deletions and chromosomal recombination. Despite these differences, pan and core genome analysis showed similar gene functions between the two strains. The phylogenomic study of the family Halomonadaceae is reported here for the first time. We found that the relationships were well resolved among every genera tested, including Chromohalobacter, Halomonas, Cobetia, Kushneria, Zymobacter, and Halotalea.