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TALEN-mediated targeted mutagenesis of fatty acid desaturase 2 (FAD2) in peanut (Arachis hypogaea L.) promotes the accumulation of oleic acid

文献类型： 外文期刊

作者： Wen, Shijie 1 ; Liu, Hao 1 ; Li, Xingyu 1 ; Chen, Xiaoping 1 ; Hong, Yanbin 1 ; Li, Haifen 2 ; Lu, Qing; Liang, Xuanqi 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Natl Ctr Oilseed Crops Improvement, South China Peanut Subctr, Crops Res Inst,Guangdong Key Lab Crop Genet Impro, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Natl Ctr Oilseed Crops Improvement, South China Peanut Subctr, Crops Res Inst,Gu

关键词： TALENs; Peanut; FAD2; Oleic acid; Genome editing

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2018 年 97 卷 1-2 期

页码：

收录情况： SCI

摘要： Transcription activator like effector nucleases (TALENs), which allow the precise editing of DNA, have already been developed and applied for genome engineering in diverse organisms. However, they are scarcely used in higher plant study and crop improvement, especially in allopolyploid plants. In the present study, we aimed to create targeted mutagenesis by TALENs in peanut. Targeted mutations in the conserved coding sequence of Arachis hypogaea fatty acid desaturase 2 (AhFAD2) were created by TALENs. Genetic stability of AhFAD2 mutations was identified by DNA sequencing in up to 9.52 and 4.11% of the regeneration plants at two different targeted sites, respectively. Mutation frequencies among AhFAD2 mutant lines were significantly correlated to oleic acid accumulation. Genetically, stable individuals of positive mutant lines displayed a 0.5-2 fold increase in the oleic acid content compared with non-transgenic controls. This finding suggested that TALEN-mediated targeted mutagenesis could increase the oleic acid content in edible peanut oil. Furthermore, this was the first report on peanut genome editing event, and the obtained high oleic mutants could serve for peanut breeding project.

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