文献类型： 外文期刊

作者： Zhang, C. B. ¹ ; Lin, C. J. ¹ ; Xu, Z. R. ² ; Chen, Z. H. ² ; Peng, B. ¹ ; Wang, P. N. ¹ ; Ding, X. Y. ¹ ; Zhao, L. M. ¹ ;

作者机构： 1.Jilin Acad Agr Sci, Natl Engn Res Ctr Soybean, Soybean Res Inst, Changchun, Jilin, Peoples R China

2.Northeast Forest Univ, Coll Life Sci, Harbin, Heilongjiang, Peoples R China

关键词： Glycine max; soybean; DNA methylation; heterosis; hybrid; parental lines

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN： 1021-4437

年卷期： 2018 年 65 卷 3 期

页码：

收录情况： SCI

摘要： To understand the methylation level and genetic delivery from parental line to their hybrid in soybean (Glycine max (L.) Merr.), four groups of parental lines and their hybrids were analyzed using methylation sensitive amplification polymorphism (MSAP). Comparisons of the different methylation distributions revealed. (1) The methylation level of the whole hybrid was lower than that of the corresponding parent lines, and the internal cytosine-methylation pattern was dominant. (2) In this study, comparing the total methylation levels in hybrids and the midparent values we assume that the larger differences in DNA methylation between parental lines lead to stronger heterosis. (3) Four demethylated and one hyper-methylated fragments were sequenced and located in the reference genome by blast analysis. These methylation changes could cause expression levels changes of a protein kinase, zinc finger protein and a mitochondrial transcript, which would affect a variety of metabolic pathways in soybean, providing an explanation for the observed heterosis.