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Pseudoalteromonas probiotics as potential biocontrol agents improve the survival of Penaeus vannamei challenged with acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus

文献类型： 外文期刊

作者： Wang, Hailiang 1 ; Wang, Chundi 1 ; Tang, Yang 1 ; Sun, Bochao 1 ; Huang, Jie 1 ; Song, Xiaoling 1 ;

作者机构： 1.hinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao 266071, Peoples R China; Chinese Acad Fishery Sci, Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China; Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, 106 Nanjing Rd, Qingdao 266071, Peoples R China

关键词： Penaeus vannamei; Vp(AHPND); Probiotics; Pseudoalteromonas; Extracellular products (ECPs)

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2018 年 494 卷

页码：

收录情况： SCI

摘要： Acute hepatopancreatic necrosis disease (AHPND) has caused a severe decline of global shrimp production and economic losses. One of the causative agents for AHPND is Vibrio parahaemolyticus (Vp(AHPND)), bearing toxin genes pirAB(vp). In this study, potential probiotic bacteria CDM8 and CDA22, isolated from the hindgut of healthy Penaeus vannamei, were rod-shaped and motile with a single polar flagellum, and they can form yellow colonies, produce oxidase and hydrolyze gelatin. The possibility of their application against Vp(AHPND) was evaluated. Both strains were found to display antagonistic activity against Vp(AHPND), which could be inhibited by catalase, and they were identified as Pseudoalteromonas spp. based on phylogenetic analysis. These two bacteria produced extracellular antibacterial compounds, according to the disc diffusion assay. Furthermore, CDM8 or CDA22 as a feed additive could (i) significantly decrease the presumptive Vibrio counts in the hindgut of shrimp (P. vannamei) after being fed for 21 days, compared to that in shrimp fed with the mixture of CDM8 and CDA22 and the commercial feed, and (ii) strikingly reduce the cumulative mortality (vs the control group: 36.7% or 76.7% vs 96.7%) of shrimp when challenged with Vp(AHPND). In addition, they could decrease copy numbers of pirA(vp) gene in shrimp, according to the quantitative PCR results. However, there is no synergistic relationship between CDM8 and CDA22 when used together as feed additives. All these results suggested that bacteria CDM8 and CDA22 were anti-Vp(AHPND) probiotic candidates and potential biological control agents against AHPND in shrimp aquaculture.

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