文献类型： 外文期刊

作者： Wang, T. D. ¹ ; Huang, T. D. ² ; Huang, H. S. ² ; Hua, Y. W. ² ;

作者机构： 1.Xinjiang Acad Agr Sci, Hainan Sanya Crop Breeding Trail Ctr, Urumqi 830002, Xinjiang, Peoples R China

2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Minist Agr, Key Lab Biol & Genet Resources Rubber Tree, Danzhou 571737, Hainan, Peoples R China

关键词： Cellular origin; secondary embryo; somaclonal variation; chromosome number; EST-SSRs

期刊名称：JOURNAL OF RUBBER RESEARCH （ 2020影响因子：0.667； 五年影响因子：0.618 ）

ISSN： 1511-1768

年卷期： 2017 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Micropropagation by secondary embryogenesis had been established in Hevea brasiliensis. However, origin and development of secondary embryos and genetic stability of the regenerated plants remained unclear Visual and cytological observations found secondary embryos mainly arose from epidermal cells of cotyledons and might originate from single cells. The number of pro-embryos (45.00) peaked after 20 days of cultivation for cell differentiation. At the maturation stage, the sum of immature embryos including globular, heart-like, and torpedo-liked secondary embryos (22.50) was approximately equal to the number of cotyledonary embryos (21.75), which was much lower than that ofpro-embryos (45.00), indicating a great number of pro-embryos were not able to convert into cotyledonary embryos under the current culture conditions. Evaluation of the genetic stability through chromosome number counting and EST-SSRs fingerprint, showed the chromosome number of the regenerated plants was similar to the mother tree (2n=36), the variation rates ofEST-SSRs loci and the regenerated plants initially increased and then decreased with multiplication cycles, and the variation rate of EST-SSRs loci was considered low (<2.61%), indicating the genome remained stable during multiplications. These results could further improve secondary embryogenesis system and inquire into its potential applications in H. brasiliensis.