Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
文献类型： 外文期刊
作者： Hou, Jinna 1 ; An, Sufang 1 ; Liu, Yongjuan 1 ; Lei, Li 2 ; Li, Baoquan 1 ;
作者机构： 1.Henan Acad Agr Sci, Crop Designing Ctr, Zhengzhou 450002, Henan, Peoples R China
2.Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA
关键词： SRAP; genetic diversity; high efficient molecular marker; upland cotton
期刊名称：CROP BREEDING AND APPLIED BIOTECHNOLOGY （ 影响因子：1.282； 五年影响因子：1.493 ）
ISSN： 1984-7033
年卷期： 2018 年 18 卷 2 期
页码：
收录情况： SCI
摘要： The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.
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