Identification and Characterization of MicroRNAs in Skin of Chinese Giant Salamander (Andrias davidianus) by the Deep Sequencing Approach
文献类型： 外文期刊
作者： Huang, Yong 1 ; Gong, Wang Bao 2 ;
作者机构： 1.Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471023, Peoples R China
2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou, Guangdong, Peoples R China
关键词： Andrias davidianus; microRNA; skin; deep sequencing; target gene
期刊名称：BIOCHEMISTRY-MOSCOW （ 影响因子：2.487； 五年影响因子：2.57 ）
ISSN： 0006-2979
年卷期： 2018 年 83 卷 6 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNA) play a pivotal role in regulating a broad range of biological processes, acting by cleaving mRNAs or by translational repression. However, the miRNAs from skin of Andrias davidianus have not been reported. In this study, a small-RNA cDNA library was constructed and sequenced from skin of A. davidianus. A total of 513 conserved miRNAs belonging to 174 families were identified. The remaining 108 miRNAs we identified were novel and likely to be skin tissue-specific but were expressed at low levels. The presence of randomly selected 15 miRNAs identified and their expression in eight different tissues from A. davidianus were validated by stem-loop qRT-PCR. For better understanding the functions of miRNAs, 129,791 predicated target genes were analyzed by GO and their pathways illustrated by KEGG pathway analyses. The results show that these identified miRNAs from A. davidianus skin are involved in a broad range of physiological functions including metabolism, growth, development, and immune responses. This study exhaustively identifies miRNAs and their target genes, which will ultimately pave the way for understanding their role in skin of A. davidianus and other amphibians. Further studies are necessary to better understand miRNA-mediated gene regulation.
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