Molecular characterization and expression analysis of TLR1 and TLR4 from the endangered fish Dabry's sturgeon (Acipenser dabryanus)
文献类型： 外文期刊
作者： Han, Panpan 1 ; Wang, Sisi 3 ; Zhang, Qihuan 2 ; Zhang, Shuhuan 4 ; Shao, Rong 2 ; Xu, Wei 2 ; Zhang, Wenbing 1 ; Xu, Qia 1 ;
作者机构： 1.Yangtze Univ, Coll Anim Sci, Jingzhou 434020, Hubei, Peoples R China
2.Yancheng Inst Technol, Jiangsu Key Lab Biochem & Biotechnol Marine Wetla, Yancheng 224051, Jiangsu, Peoples R China
3.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212003, Jiangsu, Peoples R China
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, P
关键词： Acipenser dabryanus; TLR1; TLR4; Gene expression; Immunity
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN： 0145-305X
年卷期： 2018 年 86 卷
页码：
收录情况： SCI
摘要： Toll-like receptors (TLRs) are important pattern recognition receptors (PRRs) of teleost innate immune system. However, information about TLRs is absent in Dabry's sturgeon (Acipenser dabryanus), one of the most primitive actinopterygii species. In the present study, the full lengths of adaTLR1 and adaTLR4 were cloned from Dabry's sturgeon using RT-PCR and RACE-PCR. The obtained adaTLR1 was 2957 bp in length, encoding a putative protein of 767 amino acids and adaTLR4 cDNA was 2902 bp in length, encoding a putative protein of 830 aa. Both adaTLR1 and adaTLR4 possessed several typical TLRs motifs, including signal peptides, leucine-rich repeat (LRR) motifs, a transmembrane domain and a TIR motifs. In addition, adaTLR4 contained three conserved boxes in its TIR motif, involving in TLRs signal transduction. A proline, important for LPS recognition of mammalian TLR4, was also found in adaTLR4. Physicochemical features of adaTLR1 and adaTRL4 were also analyzed. Quantitative realtime PCR showed that both transcripts were ubiquitously expressed in all 11 normal tissues selected, but they exhibited different expression patterns, with adaTLRl highly expression in heart and adaTLR4 highly in skin. Further, adaTLRl and adaTLR4 were up-regulated in the primary head-kidney leucocytes following LPS and polyI:C stimulation, indicating that both genes involved in the sturgeon immune response to LPS and polyI:C. To our best knowledge, this was the first report of these genes in sturgeon and these results provided the basis for further elucidating the ligand specificity and signaling pathway of fish TLRs. (C) 2018 Elsevier Ltd. All rights reserved.
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